Rabbit MMP16 Polyclonal Antibody | anti-MMP16 antibody

MMP16, NT (Matrix Metalloproteinase-16, MMP-16, Membrane-type Matrix Metalloproteinase 3, MT-MMP 3, MTMMP3, Membrane-type-3 Matrix Metalloproteinase, MT3-MMP, MT3MMP, MMP-X2, MMPX2) (Azide free) (HRP)

Cat. Num. AAA6487132

• Reactivity: Human, Hamster
• Applications: Immunohistochemistry, ELISA
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: MMP16; Polyclonal Antibody; NT (Matrix Metalloproteinase-16; MMP-16; Membrane-type Matrix Metalloproteinase 3; MT-MMP 3; MTMMP3; Membrane-type-3 Matrix Metalloproteinase; MT3-MMP; MT3MMP; MMP-X2; MMPX2) (Azide free) (HRP); anti-MMP16 antibody

• Host: Rabbit
• Reactivity: Human, Hamster
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MMP16. Species Crossreactivity: hamster.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-MMP16 antibody: Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human MMP16 (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(MMP16 Antibody (E169) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the MMP16 antibody detected the MMP16 protein (arrow).)

Product Categories/Family for anti-MMP16 antibody

Antibodies; Matrix Metalloproteinases

References

1. Jung, M., et al., Prostate 55(2):89-98 (2003). 2. Nagase, H., et al., J. Biol. Chem. 274(31):21491-21494 (1999). 3. Matsumoto, S., et al., Biochim. Biophys. Acta 1354(2):159-170 (1997). 4. Sato, H., et al., Genomics 39(3):412-413 (1997). 5. Mattei, M.G., et al., Genomics 40(1):168-169 (1997).