Rabbit MLN64 Polyclonal Antibody | anti-MLN64 antibody

MLN64 Antibody

Cat. Num. AAA9611903

• Gene Names: STARD3; CAB1; es64; MLN64
• Reactivity: Human, Mouse, Rat; Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
• Applications: Western Blot
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: MLN64; Polyclonal Antibody; MLN64 Antibody; CAB 1; CAB1; Es 64; Es64; FLJ41370; Metastatic lymph node gene 64 protein; Metastatic lymph node protein 64; MLN 64; Mln64; Protein CAB 1; Protein CAB1; Protein MLN 64; Protein MLN64; StAR related lipid transfer protein 3; StAR-related lipid transfer protein 3; STAR3_HUMAN; STARD 3; StARD3; START domain containing 3; START domain-containing protein 3; Steroidogenic acute regulatory protein related; anti-MLN64 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MLN64 Antibody detects endogenous levels of MLN64.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 445

• Applicable Applications for anti-MLN64 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthesized peptide derived from human MLN64.
• Tissue Specificity: Expressed in retina.
• Fragment: Fab Fragment
• Conjugate: Unconjugated
• Subcellular Location: Endosome
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from MCF-7 cells, using MLN64 Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-MLN64 antibody

Description: Sterol-binding protein that mediates cholesterol transport from the endoplasmic reticulum to endosomes (PubMed:11053434, PubMed:15930133, PubMed:22514632, PubMed:28377464). Creates contact site between the endoplasmic reticulum and late endosomes: localizes to late endosome membranes and contacts the endoplasmic reticulum via interaction with VAPA and VAPB (PubMed:24105263, PubMed:28377464). Acts as a lipid transfer protein that redirects sterol to the endosome at the expense of the cell membrane and favors membrane formation inside endosomes (PubMed:28377464). May also mediate cholesterol transport between other membranes, such as mitochondria membrane or cell membrane (PubMed:12070139, PubMed:19965586). However, such results need additional experimental evidences; probably mainly mediates cholesterol transport from the endoplasmic reticulum to endosomes (PubMed:28377464). Does not activate transcriptional cholesterol sensing (PubMed:28377464). Able to bind other lipids, such as lutein, a xanthophyll carotenoids that form the macular pigment of the retina (PubMed:21322544).

Subunit Structure: Homodimer (PubMed:15718238, PubMed:16709157). Interacts (via the MENTAL domain) with STARD3NL (PubMed:15718238, PubMed:16709157). Interacts (via FFAT motif) with VAPA (PubMed:24105263, PubMed:28377464). Interacts (via FFAT motif) with VAPB (PubMed:24105263, PubMed:28377464).

Similarity: The FFAT motif mediates interaction with VAPA and VAPB. The START domain mediates lipid-transfer between membranes. It contains a hydrophobic cavity able to accommodate one lipid molecule, thereby serving as a 'hydrophobic bridge' across the aqueous gap between donor and acceptor organelle membranes. The MENTAL domain anchors the protein in endosome membranes and exposes the START domain in the cytosol (PubMed:11053434). It binds cholesterol and mediates homotypic as well as heterotypic interactions between STARD3 and STARD3NL (PubMed:15718238, PubMed:16709157). Belongs to the STARD3 family.

NCBI and Uniprot Product Information

• NCBI GI #: 116242802
• NCBI GeneID: 10948
• UniProt Accession #: Q14849
• Molecular Weight: Observed: 50 kDa; Predicted: 51 kDa
• NCBI Official Full Name: StAR-related lipid transfer protein 3
• NCBI Official Synonym Full Names: StAR related lipid transfer domain containing 3
• NCBI Official Symbol: STARD3
• NCBI Official Synonym Symbols: CAB1; es64; MLN64
• NCBI Protein Information: stAR-related lipid transfer protein 3
• UniProt Protein Name: StAR-related lipid transfer protein 3
• Protein Family: MLN64 N-terminal domain
• UniProt Gene Name: STARD3
• UniProt Synonym Gene Names: CAB1; MLN64; MLN 64; StARD3
• UniProt Entry Name: STAR3_HUMAN

NCBI Description

This gene encodes a member of a subfamily of lipid trafficking proteins that are characterized by a C-terminal steroidogenic acute regulatory domain and an N-terminal metastatic lymph node 64 domain. The encoded protein localizes to the membranes of late endosomes and may be involved in exporting cholesterol. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Oct 2009]

Uniprot Description

STARD3: Binds and transports cholesterol. Promotes steroidogenesis in placenta and brain. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 17q11-q12

Cellular Component: mitochondrion; late endosome membrane; lysosomal membrane; cytoplasm; integral to membrane

Molecular Function: protein binding; cholesterol transporter activity; cholesterol binding

Biological Process: steroid metabolic process; cholesterol metabolic process; progesterone biosynthetic process; cholesterol transport; lipid metabolic process; mitochondrial transport