Rabbit MLL5 Polyclonal Antibody | anti-MLL5 antibody

MLL5 Polyclonal Antibody

Cat. Num. AAA3011386

• Gene Names: KMT2E; MLL5; NKp44L; ODLURO; HDCMC04P
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: MLL5; Polyclonal Antibody; MLL5 Polyclonal Antibody; HDCMC04P; Histone lysine N methyltransferase MLL5; Histone-lysine N-methyltransferase MLL5; KMT2E; Lysine N methyltransferase 2E; Lysine N-methyltransferase 2E; MGC70452; Mll5; MLL5_HUMAN; Myeloid/lymphoid or mixed lineage leukemia 5 (trithorax homolog; Drosophila); Myeloid/lymphoid or mixed lineage leukemia 5; Myeloid/lymphoid or mixed lineage leukemia protein 5; Myeloid/lymphoid or mixed-lineage leukemia protein 5; anti-MLL5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MLL5 polyclonal antibody detects endogenous levels of MLL5 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1858

• Applicable Applications for anti-MLL5 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:50-1:200
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of MLL5 polyclonal antibody)

Related Product Information for anti-MLL5 antibody

The mixed lineage leukemia (MLL) gene family comprise a group of Histone H3 lysine 4 (H3K4) methyltransferases within the larger SET1 family. The founding member MLL commonly undergoes translocations in infantile leukemia and displays increased expression in some adult myeloid leukemias. MLL2, also designated ALR, exists within a complex of proteins. MLL2 is important for mouse embryonic development and may be involved in adhesion-related cytoskeletal events affecting cell growth and survival. The MLL2 gene maps to the human locus 19q13.1, which is a frequent target of rearrangement or amplification in solid tumors. MLL3 or its paralogue MLL4 associate with activating signal cointegrator-2 (ASC-2), which regulates ligand-dependent H3K4 trimethylation and expression of LXR-target genes. MLL3 maps to a location on human chromosome 7 that is often deleted in myeloid disorders. MLL3 also exhibits higher expression in peripheral blood, placenta, pancreas, testis, and fetal thymus. MLL5 localizes to the nucleus and forms intranuclear protein complexes, which may regulate chromatin remodeling and cellular growth suppression. The gene encoding human MLL5 lies within chromosome band 7q22.3, a region deleted in cytogenetic aberrations of acute myeloid malignancies.

NCBI and Uniprot Product Information

• NCBI GI #: 91199543
• NCBI GeneID: 55904
• NCBI Accession #: NP_061152.3
• NCBI GenBank Nucleotide #: NP_061152.3
• UniProt Accession #: Q8IZD2
• Molecular Weight: ~ 95-140kDa
• NCBI Official Full Name: inactive histone-lysine N-methyltransferase 2E
• NCBI Official Synonym Full Names: lysine methyltransferase 2E (inactive)
• NCBI Official Symbol: KMT2E
• NCBI Official Synonym Symbols: MLL5; NKp44L; ODLURO; HDCMC04P
• NCBI Protein Information: inactive histone-lysine N-methyltransferase 2E
• UniProt Protein Name: Histone-lysine N-methyltransferase 2E
• UniProt Gene Name: KMT2E
• UniProt Synonym Gene Names: MLL5; Lysine N-methyltransferase 2E
• UniProt Entry Name: KMT2E_HUMAN

NCBI Description

This gene is a member of the myeloid/lymphoid or mixed-lineage leukemia (MLL) family and encodes a protein with an N-terminal PHD zinc finger and a central SET domain. Overexpression of the protein inhibits cell cycle progression. Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

MLL5: Histone methyltransferase that specifically mono- and dimethylates 'Lys-4' of histone H3 (H3K4me1 and H3K4me2). H3 'Lys- 4' methylation represents a specific tag for epigenetic transcriptional activation. Key regulator of hematopoiesis involved in terminal myeloid differentiation and in the regulation of hematopoietic stem cell (HSCs) self-renewal by a mechanism that involves DNA methylation. Plays an essential role in retinoic- acid-induced granulopoiesis by acting as a coactivator of RAR- alpha (RARA) in target gene promoters. Also acts as an important cell cycle regulator, participating in cell cycle regulatory network machinery at multiple cell cycle stages. Required to suppress inappropriate expression of S-phase-promoting genes and maintain expression of determination genes in quiescent cells. Overexpression inhibits cell cycle progression, while knockdown induces cell cycle arrest at both the G1 and G2/M phases. Belongs to the histone-lysine methyltransferase family. TRX/MLL subfamily. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Methyltransferase, protein lysine; Transcription, coactivator/corepressor; Methyltransferase; Cell cycle regulation; EC 2.1.1.43

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: nucleoplasm; intercellular bridge; intracellular membrane-bound organelle; cytoplasm; plasma membrane; nuclear speck

Molecular Function: protein binding; enzyme binding; zinc ion binding; histone lysine N-methyltransferase activity (H3-K4 specific); transcription coactivator activity

Biological Process: retinoic acid receptor signaling pathway; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; neutrophil activation; positive regulation of transcription, DNA-dependent; positive regulation of granulocyte differentiation; histone H3-K4 methylation; DNA methylation; neutrophil mediated immunity; erythrocyte differentiation; cell cycle arrest