Human Insulin-like growth factor I ELISA Kit | IGF1 elisa kit

Human Insulin-like growth factor I ELISA Kit

Cat. Num. AAA289256

• Gene Names: IGF1; MGF; IGFI; IGF-I
• Reactivity: Human
• Synonyms: Insulin-like growth factor I; Human Insulin-like growth factor I ELISA Kit; IGF-I; Mechano growth factor; MGF; Somatomedin-C; IGF1; IBP1; IGF1 elisa kit

• Reactivity: Human
• Specificity: Recombinant and natural Rat CSF-1
• Sequence Length: 153

• Samples: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates Or Other Biological Fluids
• Detection Range: 15.6-1000 pg/mL
• Sensitivity: <7.86 pg/mL
• Intra-assay Precision: Intra-Assay CV: <=6.6%
• Inter-assay Precision: Inter-Assay CV: <=8.9%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IGF1 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 11024682
• NCBI GeneID: 3479
• NCBI Accession #: NP_000609.1
• NCBI GenBank Nucleotide #: NM_000618.4
• UniProt Accession #: P05019; P01343; Q14620; B2RWM7; E9PD02
• Molecular Weight: 17,762 Da
• NCBI Official Full Name: insulin-like growth factor I isoform 4 preproprotein
• NCBI Official Synonym Full Names: insulin like growth factor 1
• NCBI Official Symbol: IGF1
• NCBI Official Synonym Symbols: MGF; IGFI; IGF-I
• NCBI Protein Information: insulin-like growth factor I
• UniProt Protein Name: Insulin-like growth factor I
• Protein Family: Insulin-like growth factor
• UniProt Gene Name: IGF1
• UniProt Synonym Gene Names: IBP1; IGF-I; MGF
• UniProt Entry Name: IGF1_HUMAN

NCBI Description

The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein. [provided by RefSeq, Sep 2015]

Uniprot Description

IGF1: The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency). IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation. Belongs to the insulin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12q23.2

Cellular Component: cell soma; extracellular region; extracellular space; insulin-like growth factor binding protein complex; plasma membrane

Molecular Function: growth factor activity; hormone activity; insulin receptor binding; insulin-like growth factor receptor binding; integrin binding; protein binding; steroid binding

Biological Process: aging; alveolus development; blood coagulation; blood vessel remodeling; branching morphogenesis of a tube; cell activation; cell motility; cell proliferation; cellular protein metabolic process; cellular response to insulin stimulus; chondroitin sulfate proteoglycan biosynthetic process; detection of mechanical stimulus involved in sensory perception; DNA replication; exocrine pancreas development; female pregnancy; glial cell differentiation; glycolate metabolic process; inner ear development; insulin-like growth factor receptor signaling pathway; mammary gland development; memory; multicellular organism growth; muscle development; muscle hypertrophy; myoblast differentiation; myoblast proliferation; myotube cell development; negative regulation of apoptosis; negative regulation of cell proliferation; negative regulation of peptidyl-tyrosine phosphorylation; phosphoinositide 3-kinase cascade; phosphoinositide-mediated signaling; platelet activation; platelet degranulation; positive regulation of activated T cell proliferation; positive regulation of cell growth; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of DNA binding; positive regulation of DNA replication; positive regulation of epithelial cell proliferation; positive regulation of fat cell differentiation; positive regulation of fibroblast proliferation; positive regulation of glucose import; positive regulation of glycogen biosynthetic process; positive regulation of glycolysis; positive regulation of granule cell precursor proliferation; positive regulation of insulin-like growth factor receptor signaling pathway; positive regulation of MAPKKK cascade; positive regulation of mitosis; positive regulation of osteoblast differentiation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein import into nucleus, translocation; positive regulation of protein kinase B signaling cascade; positive regulation of protein secretion; positive regulation of Ras protein signal transduction; positive regulation of smooth muscle cell migration; positive regulation of smooth muscle cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; positive regulation of tyrosine phosphorylation of Stat5 protein; protein kinase B signaling cascade; protein stabilization; proteoglycan biosynthetic process; Ras protein signal transduction; regulation of calcium ion transport; regulation of establishment and/or maintenance of cell polarity; regulation of gene expression; regulation of multicellular organism growth; regulation of nitric oxide biosynthetic process; regulation of translation; response to activity; response to caffeine; response to glucocorticoid stimulus; response to heat; response to hypoxia; response to lipopolysaccharide; response to nicotine; response to nutrient; satellite cell compartment self-renewal involved in skeletal muscle regeneration; signal transduction; skeletal development; water homeostasis

Disease: Insulin-like Growth Factor I Deficiency