Goat MLH3 Polyclonal Antibody | anti-MLH3 antibody

Goat anti-MLH3 Antibody

Cat. Num. AAA420072

• Gene Names: MLH3; HNPCC7
• Reactivity: Expected from sequence similarity: Human, Dog, Pig
• Applications: ELISA
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: MLH3; Polyclonal Antibody; Goat anti-MLH3 Antibody; mutL homolog 3 (E. coli); HNPCC; HNPCC7; MGC138372; S240II117; mutL (E. coli) homolog 3; mismatch repair gene MLH3; DNA mismatch repair protein MLH3; mutL homolog 3; MLH3 antibody; mutL homolog 3 (E. coli) antibody; HNPCC antibody; HNPCC7 antibody; MGC138372 antibody; S240II117 antibody; mutL (E. coli) homolog 3 antibody; mismatch repair gene MLH3 antibody; DNA mismatch repair protein MLH3 antibody; mutL homolog 3 antibody; anti-MLH3 antibody

• Host: Goat
• Reactivity: Expected from sequence similarity: Human, Dog, Pig
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: RQSLQQSMPPCEPP
• Sequence Length: 1453

• Applicable Applications for anti-MLH3 antibody: Peptide ELISA (EIA)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 2000.; Western Blot: No signal obtained yet but low background observed in HeLa lysate at up to 2ug/ml.
• Immunogen: Peptide with sequence RQSLQQSMPPCEPP, from the C Terminus of the protein sequence according to NP_001035197.1; NP_055196.2.
• Epitope: C Terminus
• Note: This antibody is expected to recognize isoform 1 (NP_001035197.1) and isoform 2 (NP_055196.2).
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 91992162
• NCBI GeneID: 27030
• NCBI Accession #: NP_001035197.1; NP_055196.2
• NCBI GenBank Nucleotide #: NM_001040108.1
• Molecular Weight: 161,021 Da
• NCBI Official Full Name: DNA mismatch repair protein Mlh3 isoform 1
• NCBI Official Synonym Full Names: mutL homolog 3
• NCBI Official Symbol: MLH3
• NCBI Official Synonym Symbols: HNPCC7
• NCBI Protein Information: DNA mismatch repair protein Mlh3
• UniProt Protein Name: DNA mismatch repair protein Mlh3
• Protein Family: DNA mismatch repair protein
• UniProt Gene Name: MLH3
• UniProt Entry Name: MLH3_HUMAN

NCBI Description

This gene is a member of the MutL-homolog (MLH) family of DNA mismatch repair (MMR) genes. MLH genes are implicated in maintaining genomic integrity during DNA replication and after meiotic recombination. The protein encoded by this gene functions as a heterodimer with other family members. Somatic mutations in this gene frequently occur in tumors exhibiting microsatellite instability, and germline mutations have been linked to hereditary nonpolyposis colorectal cancer type 7 (HNPCC7). Several alternatively spliced transcript variants have been identified, but the full-length nature of only two transcript variants has been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

MLH3: Probably involved in the repair of mismatches in DNA. Defects in MLH3 are the cause of hereditary non-polyposis colorectal cancer type 7 (HNPCC7). Mutations in more than one gene locus can be involved alone or in combination in the production of the HNPCC phenotype (also called Lynch syndrome). Most families with clinically recognized HNPCC have mutations in either MLH1 or MSH2 genes. HNPCC is an autosomal, dominantly inherited disease associated with marked increase in cancer susceptibility. It is characterized by a familial predisposition to early onset colorectal carcinoma (CRC) and extra-colonic cancers of the gastrointestinal, urological and female reproductive tracts. HNPCC is reported to be the most common form of inherited colorectal cancer in the Western world, and accounts for 15% of all colon cancers. Cancers in HNPCC originate within benign neoplastic polyps termed adenomas. Clinically, HNPCC is often divided into two subgroups. Type I: hereditary predisposition to colorectal cancer, a young age of onset, and carcinoma observed in the proximal colon. Type II: patients have an increased risk for cancers in certain tissues such as the uterus, ovary, breast, stomach, small intestine, skin, and larynx in addition to the colon. Diagnosis of classical HNPCC is based on the Amsterdam criteria: 3 or more relatives affected by colorectal cancer, one a first degree relative of the other two; 2 or more generation affected; 1 or more colorectal cancers presenting before 50 years of age; exclusion of hereditary polyposis syndromes. The term 'suspected HNPCC' or 'incomplete HNPCC' can be used to describe families who do not or only partially fulfill the Amsterdam criteria, but in whom a genetic basis for colon cancer is strongly suspected. Defects in MLH3 are a cause of colorectal cancer (CRC). Belongs to the DNA mismatch repair MutL/HexB family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; DNA repair, damage

Chromosomal Location of Human Ortholog: 14q24.3

Cellular Component: chiasma; male germ cell nucleus; nucleus; synaptonemal complex

Molecular Function: ATP binding; ATPase activity; centromeric DNA binding; chromatin binding; mismatched DNA binding; protein binding; satellite DNA binding; single-stranded DNA binding

Biological Process: female meiosis I; male meiosis; meiotic recombination; mismatch repair; protein localization; synaptonemal complex assembly

Disease: Colorectal Cancer, Hereditary Nonpolyposis, Type 7; Endometrial Cancer