Immunofluorescence (IF)
(MBS9601833 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Rabbit MKP-1/2 Polyclonal Antibody | anti-MKP-1/2 antibody
MKP-1/2 Antibody
Gene Names
DUSP1; HVH1; MKP1; CL100; MKP-1; PTPN10
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
MKP-1/2; Polyclonal Antibody; MKP-1/2 Antibody; CL 100; CL100; Dual Specificity Phosphatase 1; Dual specificity protein phosphatase 1; Dual specificity protein phosphatase hVH1; DUS1_HUMAN; DUSP 1; Dusp1; HVH1; MAP kinase phosphatase 1; Mitogen-activated protein kinase phosphatase 1; MKP-1; MKP1; Protein tyrosine phosphatase CL100; Protein-tyrosine phosphatase CL100; PTPN10; Serine/threonine specific protein phosphatase; VH1; Dual specificity phosphatase 4; Dual specificity protein phosphatase 4; Dual specificity protein phosphatase hVH2; DUS4_HUMAN; DUSP 4; Dusp4; HVH 2; HVH2; MAP kinase phosphatase 2; Mitogen activated protein kinase phosphatase 2; Mitogen-activated protein kinase phosphatase 2; MKP 2; MKP-2; MKP2; TYP; Typ1; VH 2; VH1 homologous phosphatase 2; VH2; anti-MKP-1/2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
MKP-1/2 antibody detects endogenous levels of total MKP-1/2
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
367
Applicable Applications for anti-MKP-1/2 antibody
Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IF/ICC: 1:100-1:500
IHC: 1:50-1:200
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide derived from human MKP-1/2
Subcellular Location
Nucleus
Tissue Specificity
Expressed at high levels in the lung, liver placenta and pancreas. Moderate levels seen in the heart and skeletal muscle. Lower levels found in the brain and kidney.
Predicted Cross Reactivity
Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Similarity
Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunofluorescence (IF)
(MBS9601833 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescence (IF)
(MBS9601833 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Western Blot (WB)
(Western blot analysis of MKP-1/2 expression in Jurkat whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of MKP-1/2 expression in Jurkat whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
Immunohistochemistry (IHC)
(MBS9601833 at 1/100 staining Mouse pancreas tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9601833 at 1/100 staining Mouse pancreas tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-MKP-1/2 antibody
Description: The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus.
Function: Dual specificity phosphatase that dephosphorylates MAP kinase MAPK1/ERK2 on both 'Thr-183' and 'Tyr-185', regulating its activity during the meiotic cell cycle.
Post-translational Modifications: Phosphorylation at Ser-359 and Ser-364 by MAPK1/ERK2 and MAPK3/ERK1 reduces its rate of degradation.
Similarity: Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
Function: Dual specificity phosphatase that dephosphorylates MAP kinase MAPK1/ERK2 on both 'Thr-183' and 'Tyr-185', regulating its activity during the meiotic cell cycle.
Post-translational Modifications: Phosphorylation at Ser-359 and Ser-364 by MAPK1/ERK2 and MAPK3/ERK1 reduces its rate of degradation.
Similarity: Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 41 kDa
Predicted: 40 kDa
Predicted: 40 kDa
NCBI Official Full Name
dual specificity protein phosphatase 1
NCBI Official Synonym Full Names
dual specificity phosphatase 1
NCBI Official Symbol
DUSP1
NCBI Official Synonym Symbols
HVH1; MKP1; CL100; MKP-1; PTPN10
NCBI Protein Information
dual specificity protein phosphatase 1
UniProt Protein Name
Dual specificity protein phosphatase 1
UniProt Gene Name
DUSP1
UniProt Synonym Gene Names
MAP kinase phosphatase 1; MKP-1
NCBI Description
The protein encoded by this gene is a phosphatase with dual specificity for tyrosine and threonine. The encoded protein can dephosphorylate MAP kinase MAPK1/ERK2, which results in its involvement in several cellular processes. This protein appears to play an important role in the human cellular response to environmental stress as well as in the negative regulation of cellular proliferation. Finally, the encoded protein can make some solid tumors resistant to both chemotherapy and radiotherapy, making it a target for cancer therapy. [provided by RefSeq, Aug 2017]
Uniprot Description
Dual specificity phosphatase that dephosphorylates MAP kinase MAPK1/ERK2 on both 'Thr-183' and 'Tyr-185', regulating its activity during the meiotic cell cycle.
Research Articles on MKP-1/2
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Product Notes
The MKP-1/2 dusp1 (Catalog #AAA9601833) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MKP-1/2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MKP-1/2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:50-1:200 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the MKP-1/2 dusp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MKP-1/2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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