Rabbit DUSP1/MKP1 Polyclonal Antibody | anti-DUSP1/MKP1 antibody

DUSP1/MKP1 Antibody

Cat. Num. AAA9613424

• Gene Names: DUSP1; HVH1; MKP1; CL100; MKP-1; PTPN10
• Reactivity: Human, Mouse; Predicted Reactivity: Pig (100%), Zebrafish (92%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (92%)
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Synonyms: DUSP1/MKP1; Polyclonal Antibody; DUSP1/MKP1 Antibody; CL 100; CL100; Dual Specificity Phosphatase 1; Dual specificity protein phosphatase 1; Dual specificity protein phosphatase hVH1; DUS1_HUMAN; DUSP 1; Dusp1; HVH1; MAP kinase phosphatase 1; Mitogen-activated protein kinase phosphatase 1; MKP-1; MKP1; Protein tyrosine phosphatase CL100; Protein-tyrosine phosphatase CL100; PTPN10; Serine/threonine specific protein phosphatase; VH1; anti-DUSP1/MKP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse; Predicted Reactivity: Pig (100%), Zebrafish (92%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (92%)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: DUSP1/MKP1 Antibody detects endogenous levels of total DUSP1/MKP1.; Tissue Specificity: Expressed at high levels in the lung, liver placenta and pancreas. Moderate levels seen in the heart and skeletal muscle. Lower levels found in the brain and kidney.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-DUSP1/MKP1 antibody: Western Blot (WB), Peptide ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; Peptide ELISA: 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human DUSP1/MKP1, corresponding to a region within C-terminal amino acids.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Phosphorylation at Ser-359 and Ser-364 by MAPK1/ERK2 and MAPK3/ERK1 reduces its rate of degradation.
• Similarity: Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
• Subcellular Location: Nucleus.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of DUSP1/MKP1 in lysates of HeLa, using DUSP1/MKP1 Antibody(AF4716).)

Related Product Information for anti-DUSP1/MKP1 antibody

Dual specificity phosphatase that dephosphorylates MAP kinase MAPK1/ERK2 on both 'Thr-183' and 'Tyr-185', regulating its activity during the meiotic cell cycle.

NCBI and Uniprot Product Information

• NCBI GI #: 4758204
• NCBI GeneID: 1843
• NCBI Accession #: NP_004408.1
• NCBI GenBank Nucleotide #: NM_004417.3
• UniProt Accession #: P28562; Q2V508; D3DQL9
• Molecular Weight: 39,298 Da
• NCBI Official Full Name: dual specificity protein phosphatase 1
• NCBI Official Synonym Full Names: dual specificity phosphatase 1
• NCBI Official Symbol: DUSP1
• NCBI Official Synonym Symbols: HVH1; MKP1; CL100; MKP-1; PTPN10
• NCBI Protein Information: dual specificity protein phosphatase 1; MAP kinase phosphatase 1; protein-tyrosine phosphatase CL100; dual specificity protein phosphatase hVH1; serine/threonine specific protein phosphatase; mitogen-activated protein kinase phosphatase 1
• UniProt Protein Name: Dual specificity protein phosphatase 1
• UniProt Gene Name: DUSP1
• UniProt Synonym Gene Names: CL100; MKP1; PTPN10; VH1; MAP kinase phosphatase 1; MKP-1
• UniProt Entry Name: DUS1_HUMAN

NCBI Description

The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. Furthermore, it suppresses the activation of MAP kinase by oncogenic ras in extracts of Xenopus oocytes. Thus, DUSP1 may play an important role in the human cellular response to environmental stress as well as in the negative regulation of cellular proliferation. [provided by RefSeq, Jul 2008]

Uniprot Description

MKP-1: a non-receptor, dual-specificity phosphoprotein phosphatase (DUSP). Different members of the DUSP family show distinct substrate specificities for MAPKs, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. DUSP1 specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation. It is induced in human skin fibroblasts by oxidative/heat stress and growth factors. Contains 1 rhodanese domain.

Protein type: EC 3.1.3.16; Motility/polarity/chemotaxis; EC 3.1.3.48; Protein phosphatase, dual-specificity

Chromosomal Location of Human Ortholog: 5q34

Cellular Component: cytoplasm; nucleus

Molecular Function: protein tyrosine/threonine phosphatase activity; protein binding; protein tyrosine/serine/threonine phosphatase activity; non-membrane spanning protein tyrosine phosphatase activity; MAP kinase tyrosine/serine/threonine phosphatase activity

Biological Process: response to light stimulus; negative regulation of MAP kinase activity; response to cAMP; response to retinoic acid; negative regulation of MAPKKK cascade; positive regulation of apoptosis; response to glucocorticoid stimulus; negative regulation of meiotic cell cycle; response to testosterone stimulus; protein amino acid dephosphorylation; response to estradiol stimulus; cellular response to hormone stimulus; regulation of apoptosis; response to hydrogen peroxide; endoderm formation; response to oxidative stress; response to calcium ion; negative regulation of apoptosis; inactivation of MAPK activity