Western Blot (WB)
((Figure. Western Blot; Sample: Recombinant protein.))
Rabbit anti-Human Mitogen Activated Protein Kinase Kinase 4 (MAP2K4) Polyclonal Antibody | anti-MAP2K4 antibody
Biotin-Linked Antibody to Mitogen Activated Protein Kinase Kinase 4 (MAP2K4)
Gene Names
MKK4; ARABIDOPSIS THALIANA MITOGEN-ACTIVATED PROTEIN KINASE KINASE 4; ATMEK4; ATMKK4; F19C24.26; F19C24_26; MAP KINASE KINASE 4; mitogen-activated protein kinase kinase 4; MKK4
Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry, ELISA, Western Blot, Immunocytochemistry
Purity
Affinity Chromatography.
Synonyms
Mitogen Activated Protein Kinase Kinase 4 (MAP2K4); Polyclonal Antibody; Biotin-Linked Antibody to Mitogen Activated Protein Kinase Kinase 4 (MAP2K4); anti-MAP2K4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against MAP2K4. It has been selected for its ability to recognize MAP2K4 in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography.
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Concentration
500ug/ml (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and GST-tag and its sequence is listed below. MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD WLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGS-VDEK EQKQLLMDLD VVMRSSDCPY IVQFYGALFR EGDCWICMEL MSTSFDKFYK YVYSVLDDVI PEEILGKITLATVKALNHLK ENLKIIHRDI KPSNILLDRS GNIKLCDFGI SGQLVDSIAK TRDAGCRPYM APERIDPSAS RQGYDVRSDV WSLGITLYELATGRFPYPKW NSVFDQLTQV VKGDPPQLSNSEEREFSPSF INFVNLCLTK DESKRPKYKELLKHPFILMY EERAVEVACY VCKILDQMPATPSS
Sequence Length
366
Applicable Applications for anti-MAP2K4 antibody
Immunohistochemistry (IHC) (HC-P), (IHC-F), ELISA (EIA), Western Blot (WB), ICC.
Application Notes
Western blotting: 1: 1 00-400
Immunocytochemistry in formalin fixed celis: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1: 1 00-500
Immunohistochemistry in paraffin section: 1 :50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Immunocytochemistry in formalin fixed celis: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1: 1 00-500
Immunohistochemistry in paraffin section: 1 :50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Accession No.
RPD564Hu01
Cross Reactivity
Human
Unconjugated Antibody
The unconjugated antibody version of this item is also available as catalog #MBS2004006
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
((Figure. Western Blot; Sample: Recombinant protein.))
Western Blot (WB)
((Figure. Western Blot; Sample: Recombinant protein.))
Product Categories/Family for anti-MAP2K4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
mitogen-activated protein kinase kinase 4
NCBI Official Symbol
MKK4
NCBI Official Synonym Symbols
ARABIDOPSIS THALIANA MITOGEN-ACTIVATED PROTEIN KINASE KINASE 4; ATMEK4; ATMKK4; F19C24.26; F19C24_26; MAP KINASE KINASE 4; mitogen-activated protein kinase kinase 4; MKK4
NCBI Protein Information
mitogen-activated protein kinase kinase 4
Protein Family
NCBI Description
Encodes a mitogen-activated map kinase kinase (there are nine in Arabidopsis) involved in innate immunity. This protein activates MPK3/MPK6 and early-defense genes redundantly with MKK5. In plants with both MKK5 and MKK6 levels reduced by RNAi plants, floral organs do not abscise suggestion a role for both proteins in mediating floral organ abscission.
Research Articles on MAP2K4
Similar Products
Product Notes
The MAP2K4 (Catalog #AAA2007658) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Biotin-Linked Antibody to Mitogen Activated Protein Kinase Kinase 4 (MAP2K4) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Mitogen Activated Protein Kinase Kinase 4 (MAP2K4) can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) (HC-P), (IHC-F), ELISA (EIA), Western Blot (WB), ICC. Western blotting: 1: 1 00-400 Immunocytochemistry in formalin fixed celis: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1: 1 00-500 Immunohistochemistry in paraffin section: 1 :50-200 Enzyme-linked Immunosorbent Assay: 1:100-200 Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the MAP2K4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and GST-tag and its sequence is listed below. MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD WLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGS-V DEK EQKQLLMDLD VVMRSSDCPY IVQFYGALFR EGDCWICMEL MSTSFDKFYK YVYSVLDDVI PEEILGKITL ATVKALNHLK ENLKIIHRDI KPSNILLDRS GNIKLCDFGI SGQLVDSIAK TRDAGCRPYM APERIDPSAS RQGYDVRSDV WSLGITLYEL ATGRFPYPKW NSVFDQLTQV VKGDPPQLSN SEEREFSPSF INFVNLCLTK DESKRPKYKE LLKHPFILMY EERAVEVACY VCKILDQMPA TPSS. It is sometimes possible for the material contained within the vial of "Mitogen Activated Protein Kinase Kinase 4 (MAP2K4), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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