Kinesin Family, Member 5A (KIF5A) Recombinant Protein | KIF5A recombinant protein

Recombinant Kinesin Family, Member 5A (KIF5A)

Cat. Num. AAA2010217

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Kinesin Family; Member 5A (KIF5A); Recombinant Kinesin Family; KIF5A recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGS-IHSQKQKI SFLENNLEQL TEVHKQLVRD NADLRCELPK LEKRLRATAE RVKALEGALK EAKEGAMKDK RRYQQEVDRI KEAVRYKSSG KRGHSAQIAK PVRPGHYPAS SPTNPYGTRS PECISYTNNL FQNYQNLHLQ AAPSSTSDVY FASNGATSVA PLASYQKANT DNGNATDIND NRSDLPCGYE AEDPAKLFPL HQETAAS
• Sequence Length: 1027

• Applicable Applications for KIF5A recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Ile823~Ser1027 (Accession # Q6QLM7) with two N-terminal Tags, His-tag and GST-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 47058980
• NCBI GeneID: 314906
• NCBI Accession #: NP_997688.1
• NCBI GenBank Nucleotide #: NM_212523.1
• UniProt Accession #: Q6QLM7
• Molecular Weight: 54.5kDa
• NCBI Official Full Name: kinesin heavy chain isoform 5A
• NCBI Official Synonym Full Names: kinesin family member 5A
• NCBI Official Symbol: Kif5a
• NCBI Protein Information: kinesin heavy chain isoform 5A; NKHC; neuronal kinesin heavy chain; kinesin heavy chain neuron-specific 1
• UniProt Protein Name: Kinesin heavy chain isoform 5A
• Protein Family: Kinesin
• UniProt Gene Name: Kif5a
• UniProt Synonym Gene Names: NKHC
• UniProt Entry Name: KIF5A_RAT

NCBI Description

neuron-specific member of the kinesin 5 (Kif5) family, consisting of the heavy chains of conventional kinesins; may be a microtubule motor protein; mutation in human homolog causes autosomal dominant spastic paraplegia 10 [RGD, Feb 2006]

Uniprot Description

Function: Microtubule-dependent motor required for slow axonal transport of neurofilament proteins (NFH, NFM and NFL)

By similarity. UniProtKB P33175

Subunit structure: Oligomer composed of two heavy chains and two light chains. Interacts with GRIP1

By similarity. UniProtKB P33175 UniProtKB Q61768

Subcellular location: Cytoplasm › perinuclear region. Cytoplasm › cytoskeleton. Note: Concentrated in the cell body of the neurons, particularly in the perinuclear region. Ref.2

Tissue specificity: Expressed in brain. Ref.2

Domain: Composed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.

Sequence similarities: Belongs to the kinesin-like protein family. Kinesin subfamily.Contains 1 kinesin-motor domain.