Rabbit anti-Mouse Mitogen Activated Protein Kinase Kinase 2 (MAP2K2) Polyclonal Antibody | anti-MAP2K2 antibody

Polyclonal Antibody to Mitogen Activated Protein Kinase Kinase 2 (MAP2K2)

Cat. Num. AAA2005039

• Gene Names: Map2k2; MK2; MEK2; Prkmk2; AA589381
• Reactivity: Mouse
• Applications: Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
• Purity: Affinity Chromatography
• Synonyms: Mitogen Activated Protein Kinase Kinase 2 (MAP2K2); Polyclonal Antibody; Polyclonal Antibody to Mitogen Activated Protein Kinase Kinase 2 (MAP2K2); anti-MAP2K2 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against MAP2K2. It has been selected for its ability to recognize MAP2K2 in immunohistochemical staining andwestern blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
• Concentration: 500ug/mL (varies by lot)
• Sequence: Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-LDLDEQQ RKRLEAFLTQ KAKVGELKDD DFERISELGA GNGGVVTKAR HRPSGLIMAR KLIHLEIKPA VRNQIIRELQ VLHECNSPYI VGFYGAFYSD GEISICMEHM DGGSLDQVLK EAKRIPEDIL GKVSIAVLRG LAYLREKHQI MHRDVKPSNI LVNSRGEIKL CDFGVSGQLI DSMANSFVGT RSYMSPERLQ GTHYSVQSDI WSMGLSLVEL AIGRYPIPPP DAKELEASFG RPVVDGADGE PHSVSPRPRP PGRPISVGHG MDS
• Sequence Length: 400

• Applicable Applications for anti-MAP2K2 antibody: Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Western blotting: 1:100-400; Immunocytochemistry in formalin fixed cells: 1:100-500; Immunohistochemistry in formalin fixed frozen section: 1:100-500; Immunohistochemistry in paraffin section: 1:50-200; Enzyme-linked Immunosorbent Assay: 1:100-200
• Immunogen: Recombinant MAP2K2 (Leu44~Ser313) expressed in E.coli.
• Cross Reactivity: Mouse, Rat
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2071883
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Lane1: Mouse Brain Tissue; Lane2: Mouse Kidney Tissue; Lane3: Rat Brain Tissue.)

Western Blot (WB)

(Western Blot: Sample: Recombinant MAP2K2, Mouse.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Stomach Tissue.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Liver Tissue)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Kidney Tissue)

NCBI and Uniprot Product Information

• NCBI GI #: 31560267
• NCBI GeneID: 26396
• NCBI Accession #: NP_075627.2
• NCBI GenBank Nucleotide #: NM_023138.5
• UniProt Accession #: Q63932; Q9D7B0
• Molecular Weight: 44,402 Da
• NCBI Official Full Name: dual specificity mitogen-activated protein kinase kinase 2 isoform 1
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase 2
• NCBI Official Symbol: Map2k2
• NCBI Official Synonym Symbols: MK2; MEK2; Prkmk2; AA589381
• NCBI Protein Information: dual specificity mitogen-activated protein kinase kinase 2
• UniProt Protein Name: Dual specificity mitogen-activated protein kinase kinase 2
• Protein Family: Dual specificity mitogen-activated protein kinase kinase
• UniProt Gene Name: Map2k2
• UniProt Synonym Gene Names: Mek2; Mkk2; Prkmk2; MAP kinase kinase 2; MAPKK 2; MEK 2

Uniprot Description

MEK2: a dual-specificity protein kinase of the STE7 kinase family. Phosphorylated and activated by Raf and Mos kinases. Phosphorylates a Thr and a Tyr residue in a Thr-Glu-Tyr sequence located in the activation loop of ERK2 and ERK3. A component of MAP kinase signal transduction pathways involved in mitogen growth factor signal transduction.

Protein type: EC 2.7.12.2; Kinase, protein; Protein kinase, STE; Protein kinase, dual-specificity (non-receptor); STE group; STE7 family

Chromosomal Location of Human Ortholog: 10 C1|10 39.72 cM

Cellular Component: cell cortex; cytoplasm; cytoplasmic side of plasma membrane; cytosol; early endosome; endoplasmic reticulum; focal adhesion; Golgi apparatus; intercellular junction; late endosome; membrane; microtubule; mitochondrion; nucleus; perinuclear region of cytoplasm; peroxisomal membrane

Molecular Function: ATP binding; kinase activity; MAP kinase kinase activity; metal ion binding; nucleotide binding; PDZ domain binding; protein binding; protein complex scaffold activity; protein kinase activity; protein serine/threonine kinase activator activity; protein serine/threonine kinase activity; protein serine/threonine/tyrosine kinase activity; protein tyrosine kinase activity; transferase activity

Biological Process: activation of MAPK activity; ERK1 and ERK2 cascade; heart development; MAPK cascade; negative regulation of gene expression; phosphorylation; positive regulation of axonogenesis; positive regulation of cell motility; positive regulation of ERK1 and ERK2 cascade; positive regulation of protein serine/threonine kinase activity; positive regulation of transcription, DNA-templated; protein amino acid phosphorylation; regulation of apoptosis; regulation of axon regeneration; regulation of early endosome to late endosome transport; regulation of Golgi inheritance; regulation of mitotic cell cycle; regulation of protein amino acid phosphorylation; regulation of stress-activated MAPK cascade; thymus development; thyroid gland development