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Western Blot (WB) (Western Blot; Sample: Recombinant MAPK6, Human.)

Rabbit anti-Human Mitogen Activated Protein Kinase 6 (MAPK6) Polyclonal Antibody | anti-MAPK6 antibody

Polyclonal Antibody to Mitogen Activated Protein Kinase 6 (MAPK6)

Gene Names
MAPK6; ERK3; PRKM6; p97MAPK; HsT17250
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Mitogen Activated Protein Kinase 6 (MAPK6); Polyclonal Antibody; Polyclonal Antibody to Mitogen Activated Protein Kinase 6 (MAPK6); ERK3; HsT17250; PRKM6; p97MAPK; Extracellular signal-regulated kinase 3; MAP kinase isoform p97; anti-MAPK6 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against MAPK6. It has been selected for its ability to recognize MAPK6 in immunohistochemical staining and western blotting.
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Liquid; 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Concentration
0.79mg/ml (varies by lot)
Sequence
Met1~Met316
Applicable Applications for anti-MAPK6 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
WB: 0.5-2ug/mL; 1:400-1600
IHC: 5-20ug/mL; 1:40-160
ICC: 5-20ug/mL; 1:40-160
Immunogen Sequence
Met1~Met316
Cross Reactivity
Human
Conjugation
None
Preparation and Storage
Store at 4 degree C for frequent use. Aliquot and store at -20 degree C for 12 months.
Avoid repeated freeze/thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot; Sample: Recombinant MAPK6, Human.)

Western Blot (WB) (Western Blot; Sample: Recombinant MAPK6, Human.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Cardiac Muscle Tissue; Primary Ab: 20ug/ml Rabbit Anti-Human MAPK6 Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Human Cardiac Muscle Tissue; Primary Ab: 20ug/ml Rabbit Anti-Human MAPK6 Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Product Categories/Family for anti-MAPK6 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
82,681 Da
NCBI Official Full Name
mitogen-activated protein kinase 6
NCBI Official Synonym Full Names
mitogen-activated protein kinase 6
NCBI Official Symbol
MAPK6
NCBI Official Synonym Symbols
ERK3; PRKM6; p97MAPK; HsT17250
NCBI Protein Information
mitogen-activated protein kinase 6; ERK-3; MAPK 6; p97-MAPK; MAP kinase 6; MAP kinase isoform p97; protein kinase, mitogen-activated 5; protein kinase, mitogen-activated 6; extracellular signal-regulated kinase 3; extracellular signal-regulated kinase, p9
UniProt Protein Name
Mitogen-activated protein kinase 6
UniProt Gene Name
MAPK6
UniProt Synonym Gene Names
ERK3; PRKM6; MAP kinase 6; MAPK 6; ERK-3; p97-MAPK
UniProt Entry Name
MK06_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters. [provided by RefSeq, Jul 2008]

Uniprot Description

ERK3: a protein kinase of the MAPK family. Plays a critical role in the regulation of cell growth and differentiation. Localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters.

Protein type: Kinase, protein; Protein kinase, CMGC; EC 2.7.11.24; Protein kinase, Ser/Thr (non-receptor); CMGC group; MAPK family; MAPK/ERK subfamily; ERK subfamily

Chromosomal Location of Human Ortholog: 15q21

Cellular Component: cytoplasm; nucleus

Molecular Function: MAP kinase activity; protein serine/threonine kinase activity; protein binding; protein heterodimerization activity; protein kinase binding; ATP binding

Biological Process: MAPKKK cascade; signal transduction; cell cycle; protein amino acid phosphorylation

Research Articles on MAPK6

Similar Products

Product Notes

The MAPK6 mapk6 (Catalog #AAA2112311) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Mitogen Activated Protein Kinase 6 (MAPK6) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Mitogen Activated Protein Kinase 6 (MAPK6) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). WB: 0.5-2ug/mL; 1:400-1600 IHC: 5-20ug/mL; 1:40-160 ICC: 5-20ug/mL; 1:40-160. Researchers should empirically determine the suitability of the MAPK6 mapk6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1~Met31 6. It is sometimes possible for the material contained within the vial of "Mitogen Activated Protein Kinase 6 (MAPK6), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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