Rabbit anti-Human MITF Polyclonal Antibody | anti-Mitf antibody

MITF, NT (MITF, BHLHE32, Microphthalmia-associated transcription factor, Class E basic helix-loop-helix protein 32)

Cat. Num. AAA641933

• Gene Names: Mitf; CG17469; CG40476; CG41130; CG43369; DmelCG43369; dMitf; MITF
• Reactivity: Human
• Applications: ELISA, Western Blot, Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: MITF; Polyclonal Antibody; NT (MITF; BHLHE32; Microphthalmia-associated transcription factor; Class E basic helix-loop-helix protein 32); Anti -MITF; anti-Mitf antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-Mitf antibody: ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: Suitable for use in Western Blot, Immunofluorescence, Flow Cytometry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunofluorescence: 1:10-50; Flow Cytometry: 1:10-50
• Immunogen: MITF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 8-36 amino acids from the N-terminal region of human MITF.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in K562 cell line lysates (35ug/lane) using MBS641933. This demonstrates that MBS641933 detected the MITF protein (arrow).)

Immunofluorescence (IF)

(Confocal Immunofluorescent analysis of Hela cell using MBS641933 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of K562 cells using MBS641933 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-Mitf antibody

This gene encodes a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. It regulates the differentiation and development of melanocytes retinal pigment epithelium and is also responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified.

Product Categories/Family for anti-Mitf antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 386763490
• NCBI GeneID: 3885647
• NCBI Accession #: NP_001245437.1
• NCBI GenBank Nucleotide #: NM_001258508.2
• UniProt Accession #: Q6WSQ9
• Molecular Weight: 80,877 Da
• NCBI Official Full Name: mitf, isoform D
• NCBI Official Symbol: Mitf
• NCBI Official Synonym Symbols: CG17469; CG40476; CG41130; CG43369; DmelCG43369; dMitf; MITF
• NCBI Protein Information: CG43369 gene product from transcript CG43369-RA; CG43369-PA; CG43369-PB; CG43369-PC; CG43369-PD; Mitf-PA; Mitf-PB; Mitf-PC; Mitf-PD; mitf
• UniProt Protein Name: MITF
• Protein Family: Microphthalmia-associated transcription factor
• UniProt Gene Name: Mitf
• UniProt Entry Name: Q6WSQ9_DROME

Uniprot Description

Sequence similarities: Contains 1 basic helix-loop-helix (bHLH) domain. RuleBase RU000367