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Western Blot (WB) (Western blotAll lanes: PPEF1 antibody at 1ug/mlLane 1: U251 whole cell lysateLane 2: A549 whole cell lysateLane 3: Hela whole cell lysateLane 4: Mouse gonadal tissueSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 76,70,73,45,69 kDaObserved band size: 76 kDa)

Rabbit anti-Human PPEF1 Polyclonal Antibody | anti-PPEF1 antibody

PPEF1 Antibody

Gene Names
PPEF1; PP7; PPEF; PPP7C; PPP7CA
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity Purified
Synonyms
PPEF1; Polyclonal Antibody; PPEF1 Antibody; Serine/threonine-protein phosphatase with EF-hands 1 (EC:3.1.3.16); PPEF-1; PPEF; PPP7C; Protein phosphatase with EF calcium-binding domain; Serine/threonine-protein phosphatase 7; PP7; anti-PPEF1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
Liquid
Sequence Length
653
Applicable Applications for anti-PPEF1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Recommended dilution: WB:1:500-2000, IHC:1:20-1:200
Immunogen
Recombinant human Serine/threonine-protein phosphatase with EF-hands 1 protein
Conjugation
Non-conjugated
Storage Buffer
Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-22462
Preparation and Storage
Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.

Western Blot (WB)

(Western blotAll lanes: PPEF1 antibody at 1ug/mlLane 1: U251 whole cell lysateLane 2: A549 whole cell lysateLane 3: Hela whole cell lysateLane 4: Mouse gonadal tissueSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 76,70,73,45,69 kDaObserved band size: 76 kDa)

Western Blot (WB) (Western blotAll lanes: PPEF1 antibody at 1ug/mlLane 1: U251 whole cell lysateLane 2: A549 whole cell lysateLane 3: Hela whole cell lysateLane 4: Mouse gonadal tissueSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 76,70,73,45,69 kDaObserved band size: 76 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human tonsil tissue using MBS7002469 at dilution of 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human tonsil tissue using MBS7002469 at dilution of 1:100)
Related Product Information for anti-PPEF1 antibody
May have a role in the recovery or adaptation response of photoreceptors. May have a role in development.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
68,806 Da
NCBI Official Full Name
serine/threonine-protein phosphatase with EF-hands 1 isoform 1
NCBI Official Synonym Full Names
protein phosphatase with EF-hand domain 1
NCBI Official Symbol
PPEF1
NCBI Official Synonym Symbols
PP7; PPEF; PPP7C; PPP7CA
NCBI Protein Information
serine/threonine-protein phosphatase with EF-hands 1
UniProt Protein Name
Serine/threonine-protein phosphatase with EF-hands 1
UniProt Gene Name
PPEF1
UniProt Synonym Gene Names
PPEF; PPP7C; PPEF-1; PPEF; PP7
UniProt Entry Name
PPE1_HUMAN

NCBI Description

This gene encodes a member of the serine/threonine protein phosphatase with EF-hand motif family. The protein contains a protein phosphatase catalytic domain, and at least two EF-hand calcium-binding motifs in its C terminus. Although its substrate(s) is unknown, the encoded protein has been suggested to play a role in specific sensory neuron function and/or development. This gene shares high sequence similarity with the Drosophila retinal degeneration C (rdgC) gene. Several alternatively spliced transcript variants, each encoding a distinct isoform, have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

PPEF-1: a Ser/Thr phosphatase. Contains at least two EF-hand calcium-binding motifs in its C terminus. May play a role in specific sensory neuron function and/or development. Shares high sequence similarity with the Drosophila retinal degeneration C (rdgC) gene. Several alternatively spliced isoforms have been described.

Protein type: Protein phosphatase, dual-specificity; EC 3.1.3.16; Motility/polarity/chemotaxis; Protein phosphatase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: Xp22

Cellular Component: cytosol

Molecular Function: calcium ion binding; iron ion binding; manganese ion binding; protein binding; protein serine/threonine phosphatase activity

Biological Process: detection of stimulus involved in sensory perception; phototransduction, visible light; protein amino acid dephosphorylation; regulation of rhodopsin mediated signaling; rhodopsin mediated signaling

Research Articles on PPEF1

Similar Products

Product Notes

The PPEF1 ppef1 (Catalog #AAA7002469) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PPEF1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PPEF1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Recommended dilution: WB:1:500-2000, IHC:1:20-1:200. Researchers should empirically determine the suitability of the PPEF1 ppef1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPEF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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