Rabbit anti-Human MINPP1 Polyclonal Antibody | anti-MINPP1 antibody

MINPP1, CT (MINPP1, MIPP, Multiple inositol polyphosphate phosphatase 1, 2,3-bisphosphoglycerate 3-phosphatase, Inositol (1,3,4,5)-tetrakisphosphate 3-phosphatase) (FITC)

Cat. Num. AAA6320858

• Gene Names: MINPP1; MIPP; HIPER1; MINPP2
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MINPP1; Polyclonal Antibody; CT (MINPP1; MIPP; Multiple inositol polyphosphate phosphatase 1; 2; 3-bisphosphoglycerate 3-phosphatase; Inositol (1; 3; 4; 5)-tetrakisphosphate 3-phosphatase) (FITC); 5)-tetrakisphosphate 3-phosphatase; anti-MINPP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-MINPP1 antibody: Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: MINPP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 369-348 amino acids from the C-terminal region of human MINPP1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(MINPP1 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the MINPP1 antibody detected the MINPP1 protein (arrow).)

Flow Cytometry (FC/FACS)

(MINPP1 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-MINPP1 antibody

This gene encodes multiple inositol polyphosphate phosphatase; an enzyme that removes 3-phosphate from inositol phosphate substrates. It is the only enzyme known to hydrolzye inositol pentakisphosphate and inositol hexakisphosphate. This enzyme also converts 2,3 bisphosphoglycerate (2,3-BPG) to 2-phosphoglycerate; an activity formerly thought to be exclusive to 2,3-BPG synthase/2-phosphatase (BPGM) in the Rapoport-Luebering shunt of the glycolytic pathway.

Product Categories/Family for anti-MINPP1 antibody

Antibodies; Enzymes; Phosphatase

References

Newman, A.B., et al. J. Gerontol. A Biol. Sci. Med. Sci. 65(5):478-487(2010). Cho, J., et al. Proc. Natl. Acad. Sci. U.S.A. 105(16):5998-6003(2008). Lamesch, P., et al. Genomics 89(3):307-315(2007). Grupe, A., et al. Am. J. Hum. Genet. 78(1):78-88(2006). Liu, T., et al. J. Proteome Res. 4(6):2070-2080(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 296010986
• NCBI GeneID: 9562
• NCBI Accession #: NP_001171588.1; NP_004888.2; NP_001171589.1
• NCBI GenBank Nucleotide #: NM_001178117.1
• UniProt Accession #: Q9UNW1; O95172; O95286; Q59EJ2; Q9UGA3; F5H683
• Molecular Weight: 55,051 Da
• NCBI Official Full Name: multiple inositol polyphosphate phosphatase 1 isoform 2
• NCBI Official Synonym Full Names: multiple inositol-polyphosphate phosphatase 1
• NCBI Official Symbol: MINPP1
• NCBI Official Synonym Symbols: MIPP; HIPER1; MINPP2
• NCBI Protein Information: multiple inositol polyphosphate phosphatase 1; 2,3-BPG phosphatase; ins(1,3,4,5)P(4) 3-phosphatase; 2,3-bisphosphoglycerate 3-phosphatase; multiple inositol polyphosphate phosphatase 2; inositol (1,3,4,5)-tetrakisphosphate 3-phosphatase; multiple inositol
• UniProt Protein Name: Multiple inositol polyphosphate phosphatase 1
• Protein Family: Multiple inositol polyphosphate phosphatase
• UniProt Gene Name: MINPP1
• UniProt Synonym Gene Names: MIPP; 2,3-BPG phosphatase; Ins(1,3,4,5)P(4) 3-phosphatase
• UniProt Entry Name: MINP1_HUMAN

NCBI Description

This gene encodes multiple inositol polyphosphate phosphatase; an enzyme that removes 3-phosphate from inositol phosphate substrates. It is the only enzyme known to hydrolzye inositol pentakisphosphate and inositol hexakisphosphate. This enzyme also converts 2,3 bisphosphoglycerate (2,3-BPG) to 2-phosphoglycerate; an activity formerly thought to be exclusive to 2,3-BPG synthase/2-phosphatase (BPGM) in the Rapoport-Luebering shunt of the glycolytic pathway.[provided by RefSeq, Sep 2009]

Uniprot Description

MINPP1: Acts as a phosphoinositide 5- and phosphoinositide 6- phosphatase and regulates cellular levels of inositol pentakisphosphate (InsP5) and inositol hexakisphosphate (InsP6). Also acts as a 2,3-bisphosphoglycerate 3-phosphatase, by mediating the dephosphorylation of 2,3-bisphosphoglycerate (2,3-BPG) to produce phospho-D-glycerate without formation of 3- phosphoglycerate. May play a role in bone development (endochondral ossification). Defects in MINPP1 may be involved in follicular thyroid tumors development. Belongs to the histidine acid phosphatase family. MINPP1 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; EC 3.1.3.80; Carbohydrate Metabolism - inositol phosphate; Motility/polarity/chemotaxis; EC 3.1.3.62; Hydrolase

Chromosomal Location of Human Ortholog: 10q23

Cellular Component: endoplasmic reticulum lumen; endoplasmic reticulum

Molecular Function: acid phosphatase activity; phosphohistidine phosphatase activity

Biological Process: polyphosphate metabolic process; ossification; inositol phosphate metabolic process; dephosphorylation; bone mineralization

Disease: Thyroid Carcinoma, Follicular