Rabbit anti-Human MINPP1 Polyclonal Antibody | anti-MINPP1 antibody

MINPP1, CT (MINPP1, MIPP, Multiple inositol polyphosphate phosphatase 1, 2,3-bisphosphoglycerate 3-phosphatase, Inositol (1,3,4,5)-tetrakisphosphate 3-phosphatase) (Azide free) (HRP)

Cat. Num. AAA6320859

• Gene Names: MINPP1; MIPP; HIPER1; MINPP2
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MINPP1; Polyclonal Antibody; CT (MINPP1; MIPP; Multiple inositol polyphosphate phosphatase 1; 2; 3-bisphosphoglycerate 3-phosphatase; Inositol (1; 3; 4; 5)-tetrakisphosphate 3-phosphatase) (Azide free) (HRP); 5)-tetrakisphosphate 3-phosphatase; anti-MINPP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-MINPP1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: MINPP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 369-348 amino acids from the C-terminal region of human MINPP1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(MINPP1 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the MINPP1 antibody detected the MINPP1 protein (arrow).)

Flow Cytometry (FC/FACS)

(MINPP1 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-MINPP1 antibody

This gene encodes multiple inositol polyphosphate phosphatase; an enzyme that removes 3-phosphate from inositol phosphate substrates. It is the only enzyme known to hydrolzye inositol pentakisphosphate and inositol hexakisphosphate. This enzyme also converts 2,3 bisphosphoglycerate (2,3-BPG) to 2-phosphoglycerate; an activity formerly thought to be exclusive to 2,3-BPG synthase/2-phosphatase (BPGM) in the Rapoport-Luebering shunt of the glycolytic pathway.

Product Categories/Family for anti-MINPP1 antibody

Antibodies; Enzymes; Phosphatase

References

Newman, A.B., et al. J. Gerontol. A Biol. Sci. Med. Sci. 65(5):478-487(2010). Cho, J., et al. Proc. Natl. Acad. Sci. U.S.A. 105(16):5998-6003(2008). Lamesch, P., et al. Genomics 89(3):307-315(2007). Grupe, A., et al. Am. J. Hum. Genet. 78(1):78-88(2006). Liu, T., et al. J. Proteome Res. 4(6):2070-2080(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 296010986
• NCBI GeneID: 9562
• NCBI Accession #: NP_001171588.1; NP_004888.2; NP_001171589.1
• NCBI GenBank Nucleotide #: NM_001178117.1
• UniProt Accession #: Q9UNW1; O95172; O95286; Q59EJ2; Q9UGA3; F5H683
• Molecular Weight: 55,051 Da
• NCBI Official Full Name: multiple inositol polyphosphate phosphatase 1 isoform 2
• NCBI Official Synonym Full Names: multiple inositol-polyphosphate phosphatase 1
• NCBI Official Symbol: MINPP1
• NCBI Official Synonym Symbols: MIPP; HIPER1; MINPP2
• NCBI Protein Information: multiple inositol polyphosphate phosphatase 1; 2,3-BPG phosphatase; ins(1,3,4,5)P(4) 3-phosphatase; 2,3-bisphosphoglycerate 3-phosphatase; multiple inositol polyphosphate phosphatase 2; inositol (1,3,4,5)-tetrakisphosphate 3-phosphatase; multiple inositol
• UniProt Protein Name: Multiple inositol polyphosphate phosphatase 1
• Protein Family: Multiple inositol polyphosphate phosphatase
• UniProt Gene Name: MINPP1
• UniProt Synonym Gene Names: MIPP; 2,3-BPG phosphatase; Ins(1,3,4,5)P(4) 3-phosphatase
• UniProt Entry Name: MINP1_HUMAN

NCBI Description

This gene encodes multiple inositol polyphosphate phosphatase; an enzyme that removes 3-phosphate from inositol phosphate substrates. It is the only enzyme known to hydrolzye inositol pentakisphosphate and inositol hexakisphosphate. This enzyme also converts 2,3 bisphosphoglycerate (2,3-BPG) to 2-phosphoglycerate; an activity formerly thought to be exclusive to 2,3-BPG synthase/2-phosphatase (BPGM) in the Rapoport-Luebering shunt of the glycolytic pathway.[provided by RefSeq, Sep 2009]

Uniprot Description

MINPP1: Acts as a phosphoinositide 5- and phosphoinositide 6- phosphatase and regulates cellular levels of inositol pentakisphosphate (InsP5) and inositol hexakisphosphate (InsP6). Also acts as a 2,3-bisphosphoglycerate 3-phosphatase, by mediating the dephosphorylation of 2,3-bisphosphoglycerate (2,3-BPG) to produce phospho-D-glycerate without formation of 3- phosphoglycerate. May play a role in bone development (endochondral ossification). Defects in MINPP1 may be involved in follicular thyroid tumors development. Belongs to the histidine acid phosphatase family. MINPP1 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; EC 3.1.3.80; Carbohydrate Metabolism - inositol phosphate; Motility/polarity/chemotaxis; EC 3.1.3.62; Hydrolase

Chromosomal Location of Human Ortholog: 10q23

Cellular Component: endoplasmic reticulum lumen; endoplasmic reticulum

Molecular Function: acid phosphatase activity; phosphohistidine phosphatase activity

Biological Process: polyphosphate metabolic process; ossification; inositol phosphate metabolic process; dephosphorylation; bone mineralization

Disease: Thyroid Carcinoma, Follicular