Rabbit Mindin Polyclonal Antibody | anti-W104 antibody

Mindin (W104) Polyclonal Antibody

Cat. Num. AAA3013875

• Gene Names: SPON2; DIL1; DIL-1; MINDIN; M-SPONDIN
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Mindin; Polyclonal Antibody; Mindin (W104) Polyclonal Antibody; DIL 1; DIL-1; DIL1; M spondin; SPON 2; Spon2; Spondin 2; Spondin-2; anti-W104 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Mindin (W104) polyclonal antibody detects endogenous levels of Mindin protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 331

• Applicable Applications for anti-W104 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 70-118 of Human Mindin.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of SPON2 pAb at 1:2000 dilutionLane1:PC12 whole cell lysate (40ug) Lane2:LOVO whole cell lysate (40ug) Lane3:SGC7901 whole cell lysate (40ug) Lane4:HEK293T whole cell lysate (30ug))

Related Product Information for anti-W104 antibody

The Thrombospondin proteins, Thrombospondins 1-4 and Thrombospondin 5 (also designated COMP), compose a family of glycoproteins that are involved in cell-to-cell and cell-to-matrix signaling. These extracellular, cell-surface proteins form complexes of both homo- and heteromultimers. Spondin-2, or Mindin, is also designated DIL-1 for its differential expression in cancerous and non-cancerous lung cells. Full-length SPON2 cDNA encodes a 331 amino acid protein with a domain arrangement similar to zebrafish F-Spondin and Mindin-1/Mindin-2: an FS1 domain, an FS2 domain, a hydrophobic signal sequence in the N-terminus and a Thrombospondin type I repeat. Immunoblot analysis demonstrates expression of a 42 kDa protein under reducing conditions and expression of dimers and oligomers in a concentration-dependent manner under nonreducing conditions.

NCBI and Uniprot Product Information

• NCBI GI #: 190341087
• NCBI GeneID: 10417
• NCBI Accession #: NP_001121797.1
• NCBI GenBank Nucleotide #: NP_001121797.1
• UniProt Accession #: Q9BUD6
• Molecular Weight: ~ 35kDa
• NCBI Official Full Name: spondin-2
• NCBI Official Synonym Full Names: spondin 2
• NCBI Official Symbol: SPON2
• NCBI Official Synonym Symbols: DIL1; DIL-1; MINDIN; M-SPONDIN
• NCBI Protein Information: spondin-2
• UniProt Protein Name: Spondin-2
• UniProt Gene Name: SPON2
• UniProt Synonym Gene Names: DIL1; DIL-1
• UniProt Entry Name: SPON2_HUMAN

Uniprot Description

SPON2: Cell adhesion protein that promotes adhesion and outgrowth of hippocampal embryonic neurons. Binds directly to bacteria and their components and functions as an opsonin for macrophage phagocytosis of bacteria. Essential in the initiation of the innate immune response and represents a unique pattern- recognition molecule in the ECM for microbial pathogens. Binds bacterial lipopolysaccharide (LPS).

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 4p16.3

Cellular Component: extracellular space; proteinaceous extracellular matrix

Molecular Function: lipopolysaccharide binding; metal ion binding; antigen binding

Biological Process: induction of bacterial agglutination; mast cell mediated immunity; axon guidance; innate immune response; positive regulation of interleukin-6 production; opsonization; cell adhesion; positive regulation of tumor necrosis factor production; defense response to fungus; defense response to virus