Western Blot (WB)
(MINA antibody (pAb) tested by Western blot. Detection of MINA by Western blot analysis. Positive detection of MINA in nuclear extracts (20 ?g per lane) derived from Jurkat cells (lane 1) and Raji cells (lane 2) at a 1:500 dilution.)
Rabbit anti-Human MINA Polyclonal Antibody | anti-MINA antibody
MINA antibody (pAb)
Gene Names
MINA; ROX; MDIG; NO52; MINA53
Reactivity
Human
Applications
Western Blot
Purity
Affinity Purified
Synonyms
MINA; Polyclonal Antibody; MINA antibody (pAb); ROX; MDIG; NO52; MINA53; anti-MINA antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-MINA antibody
Western Blot (WB)
Application Notes
Applications Validated:
WB: 1:500-1:2,000 dilution
Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western Blot.
WB: 1:500-1:2,000 dilution
Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western Blot.
Immunogen
This antibody was raised against a peptide within the C-terminal region of human MINA.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Western Blot (WB)
(MINA antibody (pAb) tested by Western blot. Detection of MINA by Western blot analysis. Positive detection of MINA in nuclear extracts (20 ?g per lane) derived from Jurkat cells (lane 1) and Raji cells (lane 2) at a 1:500 dilution.)
Western Blot (WB)
(MINA antibody (pAb) tested by Western blot. Detection of MINA by Western blot analysis. Positive detection of MINA in nuclear extracts (20 ?g per lane) derived from Jurkat cells (lane 1) and Raji cells (lane 2) at a 1:500 dilution.)
Related Product Information for anti-MINA antibody
Short Description: Our MINA antibody (pAb) was raised in a Rabbit host. It has been validated for use in Western blot; it has been shown to react with Human samples.
Background: MINA (MYC Induced Nuclear Antigen) is an oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. Is involved in the demethylation of trimethylated Lys-9 on histone H3 (H3K9me3), leading to an increase in ribosomal RNA expression. Also catalyzes the hydroxylation of 60S ribosomal protein L27a on His-39. May play an important role in cell growth and survival. May be involved in ribosome biogenesis, most likely during the assembly process of pre-ribosomal particles.
Background: MINA (MYC Induced Nuclear Antigen) is an oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. Is involved in the demethylation of trimethylated Lys-9 on histone H3 (H3K9me3), leading to an increase in ribosomal RNA expression. Also catalyzes the hydroxylation of 60S ribosomal protein L27a on His-39. May play an important role in cell growth and survival. May be involved in ribosome biogenesis, most likely during the assembly process of pre-ribosomal particles.
Product Categories/Family for anti-MINA antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
52,672 Da
NCBI Official Full Name
bifunctional lysine-specific demethylase and histidyl-hydroxylase MINA isoform a
NCBI Official Synonym Full Names
MYC induced nuclear antigen
NCBI Official Symbol
MINA
NCBI Official Synonym Symbols
ROX; MDIG; NO52; MINA53
NCBI Protein Information
bifunctional lysine-specific demethylase and histidyl-hydroxylase MINA; nucleolar protein 52; ribosomal oxygenase MINA; histone lysine demethylase MINA; mineral dust induced gene protein; mineral dust-induced gene protein; myc-induced nuclear antigen, 53
UniProt Protein Name
Bifunctional lysine-specific demethylase and histidyl-hydroxylase MINA
UniProt Gene Name
MINA
UniProt Synonym Gene Names
ROX
UniProt Entry Name
MINA_HUMAN
NCBI Description
MINA is a c-Myc (MYC; MIM 190080) target gene that may play a role in cell proliferation or regulation of cell growth. (Tsuneoka et al., 2002 [PubMed 12091391]; Zhang et al., 2005 [PubMed 15897898]).[supplied by OMIM, May 2008]
Uniprot Description
MINA: Involved in cellular proliferation. May play an important role in cell growth and survival. May be involved in ribosome biogenesis, most likely during the assembly process of pre-ribosomal particles. Belongs to the MINA53/NO66 family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Nucleolus
Chromosomal Location of Human Ortholog: 3q11.2
Cellular Component: transcription factor complex; cytoplasm; nucleolus; cytosol; nucleus
Molecular Function: dioxygenase activity; metal ion binding
Biological Process: transcription, DNA-dependent; ribosome biogenesis and assembly; negative regulation of transcription from RNA polymerase II promoter
Research Articles on MINA
Similar Products
Product Notes
The MINA mina (Catalog #AAA389023) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MINA antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MINA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications Validated: WB: 1:500-1:2,000 dilution Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western Blot. Researchers should empirically determine the suitability of the MINA mina for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MINA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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