Rabbit anti-Human POLR2G Polyclonal Antibody | anti-POLR2G antibody

POLR2G, CT (POLR2G, RPB7, DNA-directed RNA polymerase II subunit RPB7, DNA-directed RNA polymerase II subunit G, RNA polymerase II 19kD subunit) (Biotin)

Cat. Num. AAA6335721

• Gene Names: POLR2G; RPB7; RPB19; hRPB19; hsRPB7
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: POLR2G; Polyclonal Antibody; CT (POLR2G; RPB7; DNA-directed RNA polymerase II subunit RPB7; DNA-directed RNA polymerase II subunit G; RNA polymerase II 19kD subunit) (Biotin); RNA polymerase II 19kD subunit; anti-POLR2G antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-POLR2G antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: POLR2G antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 145-153 amino acids from the C-terminal region of human POLR2G.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(POLR2G Antibody (C-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the POLR2G antibody detected the POLR2G protein (arrow).)

Flow Cytometry (FC/FACS)

(POLR2G Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-POLR2G antibody

This gene encodes the seventh largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The protein functions in transcription initiation, and is also thought to help stabilize transcribing polyermase molecules during elongation.

Product Categories/Family for anti-POLR2G antibody

Antibodies; Enzymes; Polymerase

References

Cojocaru, M., et al. Biochem. J. 409(1):139-147(2008). Ujvari, A., et al. Nat. Struct. Mol. Biol. 13(1):49-54(2006). Meka, H., et al. Nucleic Acids Res. 33(19):6435-6444(2005). Lehner, B., et al. Genomics 83(1):153-167(2004). Zhou, M., et al. Proc. Natl. Acad. Sci. U.S.A. 100(22):12666-12671(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 219879812
• NCBI GeneID: 5436
• NCBI Accession #: NP_002687.1
• NCBI GenBank Nucleotide #: NM_002696
• UniProt Accession #: P62487; P52433; Q2M1Z4; B2R5C0
• Molecular Weight: 19,294 Da
• NCBI Official Full Name: Homo sapiens polymerase (RNA) II (DNA directed) polypeptide G (POLR2G), mRNA
• NCBI Official Synonym Full Names: polymerase (RNA) II (DNA directed) polypeptide G
• NCBI Official Symbol: POLR2G
• NCBI Official Synonym Symbols: RPB7; RPB19; hRPB19; hsRPB7
• NCBI Protein Information: DNA-directed RNA polymerase II subunit RPB7; DNA directed RNA polymerase II 19 kda polypeptide; DNA-directed RNA polymerase II 19 kDa polypeptide; DNA-directed RNA polymerase II subunit G; RNA polymerase II 19 kDa subunit; RNA polymerase II seventh subuni
• UniProt Protein Name: DNA-directed RNA polymerase II subunit RPB7
• UniProt Gene Name: POLR2G
• UniProt Synonym Gene Names: RPB7; RNA polymerase II subunit B7; RPB19
• UniProt Entry Name: RPB7_HUMAN

NCBI Description

This gene encodes the seventh largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The protein functions in transcription initiation, and is also thought to help stabilize transcribing polyermase molecules during elongation. [provided by RefSeq, Jan 2009]