Chicken anti-Human, Mouse Microtubule-Associated Protein Tau Isoform 1 Polyclonal Antibody | anti-MAPT antibody

Microtubule-Associated Protein Tau Isoform 1 (TAU)

Cat. Num. AAA628534

• Gene Names: MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; FLJ31424; MGC138549
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Microtubule-Associated Protein Tau Isoform 1; Polyclonal Antibody; Microtubule-Associated Protein Tau Isoform 1 (TAU); Anti -Microtubule-Associated Protein Tau Isoform 1 (TAU); anti-MAPT antibody

• Host: Chicken
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgY
• Specificity: Recognizes Microtubule-Associated Protein Tau Isoform 1. Species crossreactivity
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.

• Applicable Applications for anti-MAPT antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
• Application Notes: Suitable for use in Immunohistochemistry, Immunocytochemistry and Western Blot.; Dilution: Immunohistochemistry: 1:10,000-1:20,000 using 2% paraformaldehyde tissues; Immunocytochemistry: 1:10,000-1:20,000 using 2% paraformaldehyde cells; Western Blot: 1:20,000-1:50,000
• Immunogen: Two synthetic peptides corresponding to Microtubule-Associated Protein Tau Isoform 1 (KLH). Species sequence homology: mouse (P01637) and human (NP_05819).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

(Tissue section through the hippocampus of a patient with Alzheimer’s disease. Note the high concentrations of intracellular tau immunoreactivity in pyamidal neurons (brown reaction produc)t, as well as lower concentrations of reaction product in the neuropil. The slide was counterstained with hemotoxylin. Picture courtesy of Dr. Randy Woltjer, Oregon Health & Sciences University.)

Related Product Information for anti-MAPT antibody

Human Tau protein (a.k.a., "microtubule-associated protein tau isoform 1") is a 78.902 kD protein (758aa) expressed in cortical neurons of the CNS. In normal individuals, tau contains moderate degrees of phosphorylation on various serine and threonine residues scattered throughout the N-terminal half of this protein. However in various neurodegenerative diseases, such as Alzheimer's disease, Tau becomes hyperphosphorylated on these residues, leading to the pathological formation of intracellular "neurofibrillary tangles." Such tangles are hallmarks of Alzheimer's disease, as well as various other neurodegenerative diseases, collectively known as the "tauopathies."

Product Categories/Family for anti-MAPT antibody

Antibodies; Abs to Microtubule-associated Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 294862261
• NCBI GeneID: 4137
• NCBI Accession #: NP_058519.3
• NCBI GenBank Nucleotide #: NM_016835.4
• UniProt Accession #: P10636; P18518; Q14799; Q15549; Q15550; Q15551; Q1RMF6; Q53YB1; Q5CZI7; Q5XWF0; Q6QT54; Q9UDJ3; Q96N45
• Molecular Weight: 78,928 Da
• NCBI Official Full Name: microtubule-associated protein tau isoform 1
• NCBI Official Synonym Full Names: microtubule-associated protein tau
• NCBI Official Symbol: MAPT
• NCBI Official Synonym Symbols: TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; FLJ31424; MGC138549
• NCBI Protein Information: microtubule-associated protein tau; PHF-tau; OTTHUMP00000239567; OTTHUMP00000239568; OTTHUMP00000239569; OTTHUMP00000239570; OTTHUMP00000239571; paired helical filament-tau; neurofibrillary tangle protein; microtubule-associated protein tau, isoform 4; G protein beta1/gamma2 subunit-interacting factor 1
• UniProt Protein Name: Microtubule-associated protein tau
• Protein Family: Microtubule-associated protein
• UniProt Gene Name: MAPT
• UniProt Synonym Gene Names: MAPTL; MTBT1; TAU
• UniProt Entry Name: TAU_HUMAN

NCBI Description

This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy. [provided by RefSeq]

Uniprot Description

Tau: a microtubule-associated protein that regulates microtubule assembly and stability. Apparently involved in the establishment and maintenance of neuronal polarity. Mutations can result in several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. Nine differentially spliced isoforms have been described. The short isoforms allow plasticity of the cytoskeleton, whereas the longer isoforms may preferentially play a role in its stabilization.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21.1

Cellular Component: microtubule; microtubule associated complex; growth cone; axon; tubulin complex; plasma membrane; axoneme; cytosol

Molecular Function: protein binding; enzyme binding; structural constituent of cytoskeleton; microtubule binding; apolipoprotein binding; SH3 domain binding; protein kinase binding

Biological Process: apoptosis; axon extension; positive regulation of axon extension; positive regulation of microtubule polymerization; neuron migration; axon cargo transport; microtubule cytoskeleton organization and biogenesis; regulation of microtubule polymerization; adult walking behavior; mitochondrion transport along microtubule; negative regulation of intracellular transport; generation of neurons; regulation of autophagy; cell structure disassembly during apoptosis

Disease: Supranuclear Palsy, Progressive, 1; Pick Disease Of Brain; Frontotemporal Dementia; Parkinson-dementia Syndrome; Parkinson Disease, Late-onset; Frontotemporal Lobar Degeneration With Tdp43 Inclusions, Grn-related