Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using MAPT antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.)
Rabbit Tau Polyclonal Antibody | anti-MAPT antibody
MAPT Polyclonal Antibody
Gene Names
MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17
Reactivity
Human, mouse, rat
Applications
Western Blot, Immunocytochemistry, Flow Cytometry, Functional Assay
Purity
Affinity purification
Synonyms
Tau; Polyclonal Antibody; MAPT Polyclonal Antibody; MAPT; Tau ; DDPAC; FLJ31424; FTDP17; MAPTL; MGC138549; MSTD; MTBT1; MTBT2; PPND; TAU; anti-MAPT antibody
Host
Rabbit
Reactivity
Human, mouse, rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Sequence Length
776
Applicable Applications for anti-MAPT antibody
Western Blot (WB), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
Application Notes
Western Blot (WB): 1:500-1:2000
Immunocytochemistry (ICC): 1:50-1:200
Immunoprecipitation (IP): 1:50-1:100
Immunocytochemistry (ICC): 1:50-1:200
Immunoprecipitation (IP): 1:50-1:100
Immunogen
A synthetic -peptide of human MAPT
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Preparation and Storage
Store at -20 degree C or -80 degree C. Avoid freeze / thaw cycles
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using MAPT antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using MAPT antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.)
Related Product Information for anti-MAPT antibody
Tau is a heterogeneous microtubuleassociated protein that promotes and stabilizes microtubule assembly, especially in axons. Six isoforms with different aminoterminal inserts and different numbers of tandem repeats near the carboxyterminus have been identified, and tau is hyperphosphorylated at approximately 25 sites by ERK, GSK3 and CDK5 (12). Phosphorylation decreases the ability of tau to bind to microtubules. Neurofibrillary tangles are a major hallmark of Alzheimer's disease and these tangles are bundles of paired helical filaments composed of hyperphosphorylated tau. In particular, phosphorylation of Ser396 by GSK3 or CDK5 destabilizes microtubules in Alzheimer's disease. Furthermore, inclusions of tau are found in a number of other neurodegenerative diseases, collectively known as tauopathies (1,3).
References
1. Johnson, G.V. and Stoothoff, W.H. (2004) J. Cell Sci. 117, 5721-5729. 2. Hanger, D. P. et al. (1998) J. Neurochem. 71, 2465-2476. 3. Bramblett, G. T. et al. (1993) Neuron 10, 1089-1099.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
50 70kDa
NCBI Official Full Name
microtubule-associated protein tau isoform 6
NCBI Official Synonym Full Names
microtubule-associated protein tau
NCBI Official Symbol
MAPT
NCBI Official Synonym Symbols
TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17
NCBI Protein Information
microtubule-associated protein tau; PHF-tau; paired helical filament-tau; neurofibrillary tangle protein; microtubule-associated protein tau, isoform 4; G protein beta1/gamma2 subunit-interacting factor 1
UniProt Protein Name
Microtubule-associated protein tau
Protein Family
UniProt Gene Name
MAPT
UniProt Synonym Gene Names
MAPTL; MTBT1; TAU; PHF-tau
UniProt Entry Name
TAU_HUMAN
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Product Notes
The MAPT mapt (Catalog #AAA125717) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MAPT Polyclonal Antibody reacts with Human, mouse, rat and may cross-react with other species as described in the data sheet. AAA Biotech's Tau can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS). Western Blot (WB): 1:500-1:2000 Immunocytochemistry (ICC): 1:50-1:200 Immunoprecipitation (IP): 1:50-1:100. Researchers should empirically determine the suitability of the MAPT mapt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Tau, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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