Rabbit Microphthalmia Polyclonal Antibody | anti-Mitf antibody

Microphthalmia (Transcription Factor, MiTF)

Cat. Num. AAA615603

• Reactivity: Human, Rodent
• Applications: ELISA
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Microphthalmia; Polyclonal Antibody; Microphthalmia (Transcription Factor; MiTF); Anti -Microphthalmia (Transcription Factor; anti-Mitf antibody

• Host: Rabbit
• Reactivity: Human, Rodent
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MiTF. Species Crossreactivity: rodent.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, sodium azide before the addition of glycerol to 40%.

• Applicable Applications for anti-Mitf antibody: ELISA (EL/EIA)
• Application Notes: Suitable for use in ELISA.; Dilution: ELISA (Serum Titer): 1:37,500; Western Blot: Preliminary results: Human uterus 70kD
• Immunogen: Synthetic peptide cooresponding to aa 54-63, PNQPGDHVMP of Human MiTF (KLH) MW: 58.79kD
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-Mitf antibody

Mi is a basic helix-loop-helix-leucin zipper (b-HLH-ZIP) transcription factor implicated in pigmentation, mast cells and bone development. The mutation of Mi causes Waardenburg Syndrome type II in humans. In mice, a profound loss of pigmented cells in the skin eye and inner ear results, as well as osteopetrosis and defects in natural killer and mast cells. There are two known isoforms of Mi differing by 66 amino acids at the NH2 terminus. Shorter forms are expressed in melanocytes and run as two bands at 52kD and 56kD, while the longer Mi form runs as a cluster of bands at 60-70kD in osteoclasts and in B16 melonoma cells (but not other melanoma cell lines), as well as mast cells and heart (1).

Product Categories/Family for anti-Mitf antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 3319231
• NCBI GeneID: 25094
• UniProt Accession #: O88368
• Molecular Weight: 13,155 Da
• NCBI Official Full Name: microphthalmia associated transcription factor
• NCBI Official Synonym Full Names: microphthalmia-associated transcription factor
• NCBI Official Symbol: Mitf
• NCBI Protein Information: microphthalmia-associated transcription factor
• UniProt Protein Name: Microphthalmia-associated transcription factor
• Protein Family: Microphthalmia-associated transcription factor
• UniProt Gene Name: Mitf
• UniProt Entry Name: MITF_RAT

NCBI Description

basic helix-loop-helix-leucine zipper transcription factor; may play a role in osteoclast function [RGD, Feb 2006]

Uniprot Description

MITF: a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. Plays a critical role in the differentiation of various cell types including neural crest- derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium. Two isoforms are known: the M-isoform is expressed exclusively in melanocytes, while the A-isoform has a much broader range of expression. Mutations in MITF can lead to Waardenburg syndrome. Ten alternatively spliced isoforms have been described.

Protein type: Transcription factor; DNA-binding; Oncoprotein

Cellular Component: protein complex; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein dimerization activity; DNA binding; chromatin binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; camera-type eye development; cell fate commitment; Wnt receptor signaling pathway; positive regulation of transcription, DNA-dependent; osteoclast differentiation; negative regulation of transcription from RNA polymerase II promoter; bone remodeling; regulation of cell proliferation; regulation of osteoclast differentiation; regulation of transcription, DNA-dependent; pigmentation; regulation of gene expression; melanocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; protein complex assembly; cell differentiation; negative regulation of apoptosis