Rabbit anti-Human MICA Polyclonal Antibody | anti-MICA antibody

Anti-MICA Antibody (aa75-105) IHC-plus

Cat. Num. AAA249766

• Gene Names: MICA; MIC-A; PERB11.1
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, Flow Cytometry, Functional Assay
• Purity: Immunoaffinity purified
• Synonyms: MICA; Polyclonal Antibody; Anti-MICA Antibody (aa75-105) IHC-plus; Rabbit Polyclonal to Human MICA; Human MICA; HLA class I antigen; MIC-A; PERB11.1; anti-MICA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Human MICA
• Purity/Purification: Immunoaffinity purified
• Form/Format: PBS, 0.09% sodium azide
• Sequence Length: 383

• Applicable Applications for anti-MICA antibody: Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Flow Cytometry (1:10 - 1:50); IF (1:10 - 1:50); IHC-P (5 ug/ml); WB (1:1000)
• Immunogen: MICA antibody was raised against kLH-conjugated synthetic peptide from internal region of human MICA.
• Immunogen Description: KLH-conjugated synthetic peptide from internal region of human MICA.
• Immunogen Type: Synthetic peptide
• Antigen Modification: aa75-105
• Target Species: Human
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months.; For long term storage store at -20 degree C.

Immunohistochemistry (IHC)

(Anti-MICA antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunofluorescence (IF)

(Confocal immunofluorescence of MICA Antibody with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Western Blot (WB)

(Western blot of MICA Antibody in MDA-MB231 cell line lysates (35 ug/lane). MICA (arrow) was detected using the purified antibody.)

Western Blot (WB)

(MICA Antibody western blot of MDA-MB-231 cell line lysates (35 ug/lane). The MICA antibody detected the MICA protein (arrow).)

Flow Cytometry (FC/FACS)

(Flow cytometric of SK-Br-3 cells using MICA Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-MICA antibody

MICA Antibody, HLA class I antigen Antibody, MIC-A Antibody, PERB11.1 Antibody Description: MICA is the highly polymorphic major histocompatability complex class I chain-related protein A. The protein product is expressed on the cell surface, although unlike canonical class I molecules it does not seem to associate with beta-2-microglobulin. It is a ligand for the NKG2-D type II integral membrane protein receptor. The protein functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells.

NCBI and Uniprot Product Information

• NCBI GI #: 4557751
• NCBI GeneID: 100507436
• NCBI Accession #: NP_000238.1
• NCBI GenBank Nucleotide #: NM_000247.2
• UniProt Accession #: Q29983; O60917; O60918; O60919; O60920; O60933; O77956; O78150; A8KQR0; A9LRW5; A9QVE0; B1VJF1
• Molecular Weight: 31,577 Da
• NCBI Official Full Name: MHC class I polypeptide-related sequence A isoform 1 (MICA*001)
• NCBI Official Synonym Full Names: MHC class I polypeptide-related sequence A
• NCBI Official Symbol: MICA
• NCBI Official Synonym Symbols: MIC-A; PERB11.1
• NCBI Protein Information: MHC class I polypeptide-related sequence A
• UniProt Protein Name: MHC class I polypeptide-related sequence A
• Protein Family: MICAL-like protein
• UniProt Gene Name: MICA
• UniProt Synonym Gene Names: MIC-A
• UniProt Entry Name: MICA_HUMAN

NCBI Description

This gene encodes the highly polymorphic major histocompatability complex class I chain-related protein A. The protein product is expressed on the cell surface, although unlike canonical class I molecules it does not seem to associate with beta-2-microglobulin. It is a ligand for the NKG2-D type II integral membrane protein receptor. The protein functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells. Variations in this gene have been associated with susceptibility to psoriasis 1 and psoriatic arthritis, and the shedding of MICA-related antibodies and ligands is involved in the progression from monoclonal gammopathy of undetermined significance to multiple myeloma. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

MICA: Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T- cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis. Anti-MICA antibodies and ligand shedding are involved in the progression of monoclonal gammopathy of undetermined significance (MGUS)to multiple myeloma. Genetic variations in MICA may be a cause of susceptibility to psoriasis type 1 (PSORS1). Psoriasis is a common, chronic inflammatory disease of the skin with multifactorial etiology. It is characterized by red, scaly plaques usually found on the scalp, elbows and knees. These lesions are caused by abnormal keratinocyte proliferation and infiltration of inflammatory cells into the dermis and epidermis. Genetic variation in MICA is a cause of susceptibility to psoriatic arthritis (PSORAS). PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis). Belongs to the MHC class I family. MIC subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.33

Cellular Component: cell surface; cytoplasm; extracellular space; integral to plasma membrane; plasma membrane

Molecular Function: antigen binding; beta-2-microglobulin binding; natural killer cell lectin-like receptor binding; protein binding

Biological Process: antigen processing and presentation; cytolysis; defense response to bacterium; defense response to virus; gamma-delta T cell activation; immune response to tumor cell; natural killer cell mediated cytotoxicity; negative regulation of natural killer cell activation; negative regulation of natural killer cell mediated cytotoxicity; regulation of immune response; response to DNA damage stimulus; response to heat; T cell mediated cytotoxicity; viral reproduction

Disease: Psoriasis 1, Susceptibility To; Psoriatic Arthritis, Susceptibility To