Western Blot (WB)
(Anti-MIB2 Antibody (N-Term) at 1:1000 dilution + mouse heart lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 110 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Mouse MIB2 Polyclonal Antibody | anti-MIB2 antibody
MIB2 Antibody (N-Term)
Gene Names
MIB2; ZZZ5; ZZANK1
Reactivity
Mouse
Applications
Western Blot, ELISA
Synonyms
MIB2; Polyclonal Antibody; MIB2 Antibody (N-Term); E3 ubiquitin-protein ligase MIB2; 6.3.2.-; Mind bomb homolog 2; Novel zinc finger protein; Novelzin; Putative NF-kappa-B-activating protein 002N; Skeletrophin; Zinc finger ZZ type with ankyrin repeat domain protein 1; SKD; ZZANK1; anti-MIB2 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Ig
Specificity
This MIB2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 216-249 amino acids from human MIB2.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
1066
Applicable Applications for anti-MIB2 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB:~~1:1000
Subtitle
Purified Rabbit Polyclonal Antibody (Pab)
Cellular Location
Cytoplasm. Endosome. Note=Colocalizes with endosomal compartments.
Tissue Location
Expressed in skeletal muscle, and to a lesser extent in heart, brain and kidney.
Antigen Type
Synthesized Peptide
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(Anti-MIB2 Antibody (N-Term) at 1:1000 dilution + mouse heart lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 110 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Anti-MIB2 Antibody (N-Term) at 1:1000 dilution + mouse heart lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 110 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-MIB2 antibody
E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors (By similarity).
Product Categories/Family for anti-MIB2 antibody
References
Takeuchi T.,et al.Am. J. Pathol. 163:1395-1404 (2003). Takeuchi T.,et al.Submitted (JUN-2002) to the EMBL/GenBank/DDBJ databases. Matsuda A.,et al.Oncogene 22:3307-3318 (2003). Ota T.,et al.Nat. Genet. 36:40-45 (2004). Gregory S.G.,et al.Nature 441:315-321 (2006).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
109939 Da
NCBI Official Full Name
E3 ubiquitin-protein ligase MIB2 isoform 2
NCBI Official Synonym Full Names
mindbomb E3 ubiquitin protein ligase 2
NCBI Official Symbol
MIB2
NCBI Official Synonym Symbols
ZZZ5; ZZANK1
NCBI Protein Information
E3 ubiquitin-protein ligase MIB2
UniProt Protein Name
E3 ubiquitin-protein ligase MIB2
Protein Family
UniProt Gene Name
MIB2
UniProt Synonym Gene Names
SKD; ZZANK1; Novelzin
UniProt Entry Name
MIB2_HUMAN
Uniprot Description
MIB2: E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. 9 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 6.3.2.-; Ubiquitin conjugating system; Ubiquitin ligase; Ligase
Chromosomal Location of Human Ortholog: 1p36.33
Cellular Component: cytoplasm; cytosol; early endosome; ubiquitin ligase complex
Molecular Function: actin binding; ligase activity; protein binding; signal transducer activity; ubiquitin-protein ligase activity; zinc ion binding
Biological Process: Notch signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB cascade; protein polyubiquitination
Research Articles on MIB2
Similar Products
Product Notes
The MIB2 mib2 (Catalog #AAA9217208) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MIB2 Antibody (N-Term) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MIB2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB:~~1:1000. Researchers should empirically determine the suitability of the MIB2 mib2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MIB2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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