Rabbit anti-Human GPC1 Polyclonal Antibody | anti-GPC1 antibody

GPC1 Antibody

Cat. Num. AAA9415607

• Gene Names: GPC1; glypican
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Antigen Affinity Purification
• Synonyms: GPC1; Polyclonal Antibody; GPC1 Antibody; glypican; anti-GPC1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total GPC1 protein.
• Purity/Purification: Antigen Affinity Purification
• Form/Format: Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Concentration: 1.5 mg/ml (varies by lot)
• Sequence Length: 558

• Applicable Applications for anti-GPC1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western Blot: 1:200-1:1000; Immunohistochemistry: 1:25-1:100
• Immunogen: Synthetic peptide of human GPC1
• Immunogen Type: Peptide
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Gel: 6%SDS-PAGE Lysate: 40 μ g Lane 1-2: HEPG2 and Jurkat cell Primary antibody: 1/350 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 40 seconds)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissue using at dilution 1/25.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue using at dilution 1/25.)

Related Product Information for anti-GPC1 antibody

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation.

Product Categories/Family for anti-GPC1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 167001141
• NCBI GeneID: 2817
• NCBI Accession #: NP_002072.2
• NCBI GenBank Nucleotide #: NM_002081.2
• UniProt Accession #: P35052; Q53QM4; B3KTD1
• Molecular Weight: 31,931 Da
• NCBI Official Full Name: glypican-1
• NCBI Official Synonym Full Names: glypican 1
• NCBI Official Symbol: GPC1
• NCBI Official Synonym Symbols: glypican
• NCBI Protein Information: glypican-1
• UniProt Protein Name: Glypican-1
• Protein Family: Glypican
• UniProt Gene Name: GPC1
• UniProt Entry Name: GPC1_HUMAN

Uniprot Description

GPC1: Cell surface proteoglycan that bears heparan sulfate. Binds, via the heparan sulfate side chains, alpha-4 (V) collagen and participates in Schwann cell myelination. May act as a catalyst in increasing the rate of conversion of prion protein PRPN(C) to PRNP(Sc) via associating (via the heparan sulfate side chains) with both forms of PRPN, targeting them to lipid rafts and facilitating their interaction. Required for proper skeletal muscle differentiation by sequestering FGF2 in lipid rafts preventing its binding to receptors (FGFRs) and inhibiting the FGF-mediated signaling. Associates (via the heparan sulfate side chains) with fibrillar APP-beta amyloid peptides in primitive and classic amyloid plaques and may be involved in the deposition of these senile plaques in the Alzheimer disease (AD) brain. Misprocessing of GPC1 is found in fibroblasts of patients with Niemann-Pick Type C1 disease. This is due to the defective deaminative degradation of heparan sulfate chains. Belongs to the glypican family.

Protein type: Extracellular matrix; Membrane protein, GPI anchor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2q35-q37

Cellular Component: Golgi lumen; integral to plasma membrane; lipid raft; lysosomal lumen; plasma membrane; proteinaceous extracellular matrix

Molecular Function: copper ion binding; fibroblast growth factor binding; laminin binding

Biological Process: axon guidance; glycosaminoglycan biosynthetic process; glycosaminoglycan catabolic process; glycosaminoglycan metabolic process; heparan sulfate proteoglycan catabolic process; myelin formation; negative regulation of fibroblast growth factor receptor signaling pathway; retinoid metabolic process; Schwann cell differentiation