ELISA (EIA)
(ELISA plate was coated with serially diluted Mouse IgG2c-UNLB . Immunoglobulin was detected with Goat Anti-Mouse IgG1, Human ads-BIOT , Goat Anti-Mouse IgG2c, Human ads-BIOT , Goat Anti-Mouse IgG2b, Human ads-BIOT , Goat Anti-Mouse IgG3, Human ads-BIOT , Goat Anti-Mouse IgA-BIOT , and Goat Anti-Mouse IgM, Human ads-BIOT followed by Streptavidin-HRP and quantified.)
IgG2c isotype control
Mouse IgG2c-LE/AF
Applications
ELISA, Flow Cytometry, Functional Assay
Synonyms
IgG2c; Mouse IgG2c-LE/AF; IgG2c isotype control
Host
Mouse
Isotype
Mouse IgG2c kappa
Clone Number
6.3
Form/Format
Phosphate buffered saline, pH7.4
Concentration
0.5mg/mL (varies by lot)
Applicable Applications for IgG2c isotype control
ELISA (EIA), Flow Cytometry (FC/FACS)
Application Notes
Applications for relevant formats of this clone include-
Flow Cytometry- Quality tested
ELISA- Quality tested 1-7
Flow Cytometry- Quality tested
ELISA- Quality tested 1-7
Conjugation
LE/AF (Low Endotoxin/Azide Free)
Preparation and Storage
Store at 2-8 degree C; handle under aseptic conditions
ELISA (EIA)
(ELISA plate was coated with serially diluted Mouse IgG2c-UNLB . Immunoglobulin was detected with Goat Anti-Mouse IgG1, Human ads-BIOT , Goat Anti-Mouse IgG2c, Human ads-BIOT , Goat Anti-Mouse IgG2b, Human ads-BIOT , Goat Anti-Mouse IgG3, Human ads-BIOT , Goat Anti-Mouse IgA-BIOT , and Goat Anti-Mouse IgM, Human ads-BIOT followed by Streptavidin-HRP and quantified.)
ELISA (EIA)
(ELISA plate was coated with serially diluted Mouse IgG2c-UNLB . Immunoglobulin was detected with Goat Anti-Mouse IgG1, Human ads-BIOT , Goat Anti-Mouse IgG2c, Human ads-BIOT , Goat Anti-Mouse IgG2b, Human ads-BIOT , Goat Anti-Mouse IgG3, Human ads-BIOT , Goat Anti-Mouse IgA-BIOT , and Goat Anti-Mouse IgM, Human ads-BIOT followed by Streptavidin-HRP and quantified.)
References
1. Haas KM, Watanabe R, Matsushita T, Nakashima H, Ishiura N, Okochi H, et al. Protective and pathogenic roles for B cells during systemic autoimmunity in NZB/W F1 mice. J Immunol. 2010;184:4789-800. (ELISA)
2. Cardilo-Reis L, Gruber S, Schreier SM, Drechsler M, Papac-Milicevic N, Weber C, et al. Interleukin-13 protects from atherosclerosis and modulates plaque composition by skewing the macrophage phenotype. EMBO Mol Med. 2012;4:1072-86. (ELISA)
3. Allan RS, Zueva E, Cammas F, Schreiber HA, Masson V, Belz GT, et al. An epigenetic silencing pathway controlling T helper 2 cell lineage commitment. Nature. 2012;487:249-53. (ELISA)
4. Donius LR, Handy JM, Weis JJ, Weis JH. Optimal germinal center B cell activation and T-dependent antibody responses require expression of the mouse complement receptor Cr1. J Immunol. 2013;191:434-47. (ELISA)
5. Vogelzang A, McGuire HM, Liu SM, Gloss B, Mercado K, Earls P, et al. IL-21 contributes to fatal inflammatory disease in the absence of Foxp3+ T regulatory cells. J Immunol. 2014;192:1404-14. (ELISA)
6. Halemano K, Guo K, Heilman KJ, Barrett BS, Smith DS, Hasenkrug KJ, et al. Immunoglobulin somatic hypermutation by APOBEC3/Rfv3 during retroviral infection. Proc Natl Acad Sci USA. 2014;111:7759-64. (ELISA)
7. Pioli PD, Chen X, Weis JJ, Weis JH. Fatal autoimmunity results from the conditional deletion of Snai2 and Snai3. Cell Immunol. 2015;295:1-18. (ELISA)
2. Cardilo-Reis L, Gruber S, Schreier SM, Drechsler M, Papac-Milicevic N, Weber C, et al. Interleukin-13 protects from atherosclerosis and modulates plaque composition by skewing the macrophage phenotype. EMBO Mol Med. 2012;4:1072-86. (ELISA)
3. Allan RS, Zueva E, Cammas F, Schreiber HA, Masson V, Belz GT, et al. An epigenetic silencing pathway controlling T helper 2 cell lineage commitment. Nature. 2012;487:249-53. (ELISA)
4. Donius LR, Handy JM, Weis JJ, Weis JH. Optimal germinal center B cell activation and T-dependent antibody responses require expression of the mouse complement receptor Cr1. J Immunol. 2013;191:434-47. (ELISA)
5. Vogelzang A, McGuire HM, Liu SM, Gloss B, Mercado K, Earls P, et al. IL-21 contributes to fatal inflammatory disease in the absence of Foxp3+ T regulatory cells. J Immunol. 2014;192:1404-14. (ELISA)
6. Halemano K, Guo K, Heilman KJ, Barrett BS, Smith DS, Hasenkrug KJ, et al. Immunoglobulin somatic hypermutation by APOBEC3/Rfv3 during retroviral infection. Proc Natl Acad Sci USA. 2014;111:7759-64. (ELISA)
7. Pioli PD, Chen X, Weis JJ, Weis JH. Fatal autoimmunity results from the conditional deletion of Snai2 and Snai3. Cell Immunol. 2015;295:1-18. (ELISA)
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Product Notes
The IgG2c (Catalog #AAA679375) is an Isotype Control produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's IgG2c can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow Cytometry (FC/FACS). Applications for relevant formats of this clone include- Flow Cytometry- Quality tested ELISA- Quality tested 1-7. Researchers should empirically determine the suitability of the IgG2c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgG2c, Isotype Control" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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