Rabbit anti-Human MGAT5 Polyclonal Antibody | anti-MGAT5 antibody

MGAT5, CT (MGAT5, GGNT5, Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A, Alpha-mannoside beta-1,6-N-acetylglucosaminyltransferase, GlcNAc-T V, Mannoside acetylglucosaminyltransferase 5, N-acetylglucosaminyl-transferase V) (FITC)

Cat. Num. AAA6320638

• Gene Names: MGAT5; GNT-V; GNT-VA
• Reactivity: Human
• Applications: Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: MGAT5; Polyclonal Antibody; CT (MGAT5; GGNT5; Alpha-1; 6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A; Alpha-mannoside beta-1; 6-N-acetylglucosaminyltransferase; GlcNAc-T V; Mannoside acetylglucosaminyltransferase 5; N-acetylglucosaminyl-transferase V) (FITC); N-acetylglucosaminyl-transferase V; anti-MGAT5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MGAT5.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-MGAT5 antibody: Western Blot (WB), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 652-680 in the C-terminal region of human MGAT5, UniProt Accession #Q09328.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in NCI-H460 cell line lysates (35ug/lane) usingMBS6006194. This demonstrates that MBS6006194 detected the MGAT5 protein (arrow).)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of NCI-H460 cells using MBS6006194 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-MGAT5 antibody

This gene encodes mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase, a glycosyltransferase involved in the synthesis of protein-bound and lipid-bound oligosaccharides. Alterations of the oligosaccharides on cell surface glycoproteins cause significant changes in the adhesive or migratory behavior of a cell. Increase in the encoded protein's activity may correlate with the progression of invasive malignancies.

Product Categories/Family for anti-MGAT5 antibody

Antibodies; Enzymes; Glycosyltransferase

References

1. Dick, D.M., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 153B (6), 1179-1188 (2010). 2. Brynedal, B., et al. J. Neuroimmunol. 220 (1-2), 120-124 (2010). 3. Benson, V., et al. Int. Immunol. 22(3):167-177 (2010). 4. Wang, C., et al. J. Cell. Biochem. 109(1):113-123 (2010). 5. Ding, H., et al. Stroke 41(1):177-180 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 4505167
• NCBI GeneID: 4249
• NCBI Accession #: NP_002401.1
• NCBI GenBank Nucleotide #: NM_002410.4
• UniProt Accession #: Q09328; D3DP70
• Molecular Weight: 84,543 Da
• NCBI Official Full Name: alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A
• NCBI Official Synonym Full Names: mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase
• NCBI Official Symbol: MGAT5
• NCBI Official Synonym Symbols: GNT-V; GNT-VA
• NCBI Protein Information: alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A
• UniProt Protein Name: Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A
• Protein Family: Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase
• UniProt Gene Name: MGAT5
• UniProt Synonym Gene Names: GGNT5; GNT-V

NCBI Description

The protein encoded by this gene belongs to the glycosyltransferase family. It catalyzes the addition of beta-1,6-N-acetylglucosamine to the alpha-linked mannose of biantennary N-linked oligosaccharides present on the newly synthesized glycoproteins. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides. Alterations of the oligosaccharides on cell surface glycoproteins cause significant changes in the adhesive or migratory behavior of a cell. Increase in the activity of this enzyme has been correlated with the progression of invasive malignancies. [provided by RefSeq, Oct 2011]

Uniprot Description

Catalyzes the addition of N-acetylglucosamine in beta 1-6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides.