Bovine Potassium voltage-gated channel subfamily B member 2 ELISA Kit | KCNB2 elisa kit

Bovine Potassium voltage-gated channel subfamily B member 2 ELISA Kit

Cat. Num. AAA288293

• Reactivity: Bovine
• Synonyms: Potassium voltage-gated channel subfamily B member 2; Bovine Potassium voltage-gated channel subfamily B member 2 ELISA Kit; KCNB2 elisa kit

• Reactivity: Bovine
• Specificity: This assay recognizes recombinant and natural Bovine Potassium voltage-gated channel subfamily B member 2. No significant cross-reactivity or interference was observed.
• Sequence Length: 911

• Samples: Serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
• Assay Type: Competitive
• Detection Range: 1.56-100 ng/ml.
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for KCNB2 elisa kit

Principle of the Assay: The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Potassium voltage-gated channel subfamily B member 2, During the reaction, Potassium voltage-gated channel subfamily B member 2 in the sample or standard competes with a fixed amount of biotin-labeled Potassium voltage-gated channel subfamily B member 2 for sites on a pre-coated Monoclonal antibody specific to Potassium voltage-gated channel subfamily B member 2. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Potassium voltage-gated channel subfamily B member 2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 66792908
• NCBI GeneID: 535990
• NCBI Accession #: NP_001019734.1
• NCBI GenBank Nucleotide #: NM_001024563.1
• UniProt Accession #: Q4ZHA6
• Molecular Weight: 102,276 Da
• NCBI Official Full Name: potassium voltage-gated channel subfamily B member 2
• NCBI Official Symbol: KCNB2
• NCBI Protein Information: potassium voltage-gated channel subfamily B member 2
• UniProt Protein Name: Potassium voltage-gated channel subfamily B member 2
• Protein Family: Potassium voltage-gated channel subfamily
• UniProt Gene Name: KCNB2
• UniProt Entry Name: KCNB2_BOVIN

Uniprot Description

Voltage-gated potassium channel that mediates transmembrane potassium transport in excitable membranes, primarily in the brain and smooth muscle cells. Channels open or close in response to the voltage difference across the membrane, letting potassium ions pass in accordance with their electrochemical gradient. Homotetrameric channels mediate a delayed-rectifier voltage-dependent outward potassium current that display rapid activation and slow inactivation in response to membrane depolarization. Can form functional homotetrameric and heterotetrameric channels that contain variable proportions of KCNB1; channel properties depend on the type of alpha subunits that are part of the channel. Can also form functional heterotetrameric channels with other alpha subunits that are non-conducting when expressed alone, such as KCNS1 and KCNS2, creating a functionally diverse range of channel complexes. In vivo, membranes probably contain a mixture of heteromeric potassium channel complexes, making it difficult to assign currents observed in intact tissues to any particular potassium channel family member. Contributes to the delayed-rectifier voltage-gated potassium current in cortical pyramidal neurons and smooth muscle cells.