Rabbit anti-Human MGAT4C Polyclonal Antibody | anti-MGAT4C antibody

MGAT4C, CT (MGAT4C, Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase C, N-acetylglucosaminyltransferase IV homolog, N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase IVc, UDP-N-acetylglucosamine: alpha-1,3-D-man

Cat. Num. AAA6320625

• Gene Names: MGAT4C; GNTIVH; HGNT-IV-H
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MGAT4C; Polyclonal Antibody; CT (MGAT4C; Alpha-1; 3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase C; N-acetylglucosaminyltransferase IV homolog; N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase IVc; UDP-N-acetylglucosamine: alpha-1; 3-D-man; 3-D-mannoside beta-1; 4-N-acetylglucosaminyltransferase IVc; anti-MGAT4C antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-MGAT4C antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: MGAT4C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 302-332 amino acids from the C-terminal region of human MGAT4C.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(MGAT4C Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the MGAT4C antibody detected the MGAT4C protein (arrow).)

Immunohistochemistry (IHC)

(MGAT4C Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MGAT4C Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-MGAT4C antibody

Glycosyltransferase that participates in the transfer of N-acetylglucosamine (GlcNAc) to the core mannose residues of N-linked glycans. Catalyzes the formation of the GlcNAcbeta1-4 branch on the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans. Essential for the production of tri-and tetra-antennary N-linked sugar chains (By similarity). Does not catalyze the transfer of GlcNAc to the Manalpha1-6 arm to form GlcNAcBeta1-4Manalpha1-6 linkage ('GnT-VI' activity).

Product Categories/Family for anti-MGAT4C antibody

Antibodies; Enzymes; Glycosyltransferase

References

Rose, J. Phd, et al. Mol. Med. (2010). In press: Trynka, G., et al. Gut 58(8):1078-1083(2009). Furukawa, T., et al. J. Hum. Genet. 44(6):397-401(1999). Thomas, B.A. Br. J. Dermatol. 80 (6), 413 (1968).

NCBI and Uniprot Product Information

• NCBI GI #: 166197698
• NCBI GeneID: 25834
• NCBI Accession #: NP_037376.2
• NCBI GenBank Nucleotide #: NM_013244.3
• UniProt Accession #: Q9UBM8; Q4G199; Q9UIU5
• Molecular Weight: 56,061 Da
• NCBI Official Full Name: alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase C
• NCBI Official Synonym Full Names: MGAT4 family, member C
• NCBI Official Symbol: MGAT4C
• NCBI Official Synonym Symbols: GNTIVH; HGNT-IV-H
• NCBI Protein Information: alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase C; gnT-IVc; glcNAc-T IVc; N-acetylglucosaminyltransferase IVc; N-acetylglucosaminyltransferase IV homolog; N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase IVc
• UniProt Protein Name: Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase C
• Protein Family: Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase
• UniProt Gene Name: MGAT4C
• UniProt Synonym Gene Names: hGnT-IV-H; GlcNAc-T IVc; GnT-IVc; N-acetylglucosaminyltransferase IVc
• UniProt Entry Name: MGT4C_HUMAN

Uniprot Description

MGAT4C: Glycosyltransferase that participates in the transfer of N-acetylglucosamine (GlcNAc) to the core mannose residues of N- linked glycans. Catalyzes the formation of the GlcNAcbeta1-4 branch on the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans. Essential for the production of tri- and tetra-antennary N-linked sugar chains. Does not catalyze the transfer of GlcNAc to the Manalpha1-6 arm to form GlcNAcBeta1-4Manalpha1-6 linkage ('GnT-VI' activity). Belongs to the glycosyltransferase 54 family.

Protein type: Transferase; EC 2.4.1.145; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12q21

Cellular Component: Golgi membrane; integral to membrane

Molecular Function: alpha-1,3-mannosylglycoprotein 4-beta-N-acetylglucosaminyltransferase activity; metal ion binding

Biological Process: cellular protein metabolic process; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification