Rabbit anti-Human, Mouse EphB1 Polyclonal Antibody | anti-EphB1 antibody

EphB1, CT (Ephrin Type-B Receptor 1, Tyrosine-protein Kinase Receptor EPH-2, NET, EPH-like Kinase 6, EK6, hEK6, HEK-6, EPHT2) (PE)

Cat. Num. AAA6474387

• Gene Names: EPHB1; ELK; NET; Hek6; EPHT2
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: EphB1; Polyclonal Antibody; CT (Ephrin Type-B Receptor 1; Tyrosine-protein Kinase Receptor EPH-2; NET; EPH-like Kinase 6; EK6; hEK6; HEK-6; EPHT2) (PE); anti-EphB1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human EphB1. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-EphB1 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human EphB1 (KLH).
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-EphB1 Pab in mouse brain tissue. EphB1 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of EphB1 in gastric cancer tissues. a EphB1 protein expressed in normal mucosa at the glandular compartment and in a decreasing gradient from the glandular compartment to the foveolar compartment. b EphB1 protein focally positively stained in well-differentiated gastric cancer cells. c EphB1 protein is focally positive in poorly differentiated gastric cancer cells. d Loss of EphB1 expression in gastric cancer cells.(Provided by Jian-dong Wang,Department of Pathology Nanjing Jinling Hospital/Nanjing University School of Medicine))

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(EPHB1 Antibody (H970) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the EPHB1 antibody detected the EPHB1 protein (arrow).)

Product Categories/Family for anti-EphB1 antibody

Antibodies; Tyrosine Kinases

References

1. Prevost, N., et al., Proc. Natl. Acad. Sci. USA 99(14):9219-9224 (2002). 2. Wilkinson, D.G., Nat Rev Neurosci 2(3):155-164 (2001). 3. Xu, Q., et al., Philos. Trans. R. Soc. Lond., B, Biol. Sci. 355(1399):993-1002 (2000). 4. Holder, N., et al., Development 126(10):2033-2044 (1999). 5. Stein, E., et al., J. Biol. Chem. 273(3):1303-1308 (1998). 6.Zhen Sheng, et al. Pathobiology. 2008;75(5):274-80. (E-pub) 7.Jian-dong Wang, et al. Oncology 2007;73:238-245.

NCBI and Uniprot Product Information

• NCBI GI #: 4758284
• NCBI GeneID: 2047
• NCBI Accession #: NP_004432.1
• NCBI GenBank Nucleotide #: NM_004441.4
• UniProt Accession #: P54762; O43569; O95142; O95143; Q0VG87; A8K593
• Molecular Weight: 109,885 Da
• NCBI Official Full Name: ephrin type-B receptor 1
• NCBI Official Synonym Full Names: EPH receptor B1
• NCBI Official Symbol: EPHB1
• NCBI Official Synonym Symbols: ELK; NET; Hek6; EPHT2
• NCBI Protein Information: ephrin type-B receptor 1; EK6; EPH-like kinase 6; eph tyrosine kinase 2; soluble EPHB1 variant 1; tyrosine-protein kinase receptor EPH-2; neuronally-expressed EPH-related tyrosine kinase
• UniProt Protein Name: Ephrin type-B receptor 1
• Protein Family: Ephrin type-B receptor
• UniProt Gene Name: EPHB1
• UniProt Synonym Gene Names: ELK; EPHT2; HEK6; NET; EK6; hEK6; NET
• UniProt Entry Name: EPHB1_HUMAN

NCBI Description

Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members. [provided by RefSeq, Jul 2008]

Uniprot Description

EphB1: a receptor tyrosine kinase of the Eph family. Receptor for members of the ephrin-B family: ephrin-B1, -B2 and -B3. The Eph receptor tyrosine kinase family, the largest in the tyrosine kinase group, has fourteen members. They bind membrane-anchored ligands, ephrins, at sites of cell-cell contact, regulating the repulsion and adhesion of cells that underlie the establishment, maintenance, and remodeling of patterns of cellular organization. Eph signals are particularly important in regulating cell adhesion and cell migration during development, axon guidance, homeostasis and disease. EphA receptors bind to GPI-anchored ephrin-A ligands, while EphB receptors bind to ephrin-B proteins that have a transmembrane and cytoplasmic domain. Interactions between EphB receptor kinases and ephrin-B proteins transduce signals bidirectionally, signaling to both interacting cell types. Eph receptors and ephrins also regulate the adhesion of endothelial cells and are required for the remodeling of blood vessels. The ligand-activated form of EphB1 interacts with GRB2, GRB10 and NCK through their respective SH2 domains. Four alternatively spliced isoforms are known.

Protein type: Protein kinase, tyrosine (receptor); Kinase, protein; Protein kinase, TK; Membrane protein, integral; EC 2.7.10.1; TK group; Eph family

Chromosomal Location of Human Ortholog: 3q21-q23

Cellular Component: early endosome membrane; integral to plasma membrane; axon; dendrite; extracellular region; plasma membrane; cytosol; lipid raft

Molecular Function: protein binding; transmembrane-ephrin receptor activity; axon guidance receptor activity; ATP binding

Biological Process: positive regulation of synaptogenesis; axon guidance; camera-type eye morphogenesis; peptidyl-tyrosine phosphorylation; neurogenesis; establishment of cell polarity; protein amino acid autophosphorylation; ephrin receptor signaling pathway; central nervous system projection neuron axonogenesis; angiogenesis; optic nerve morphogenesis; detection of temperature stimulus involved in sensory perception of pain; regulation of JNK cascade; cell-substrate adhesion; retinal ganglion cell axon guidance