Mouse anti-Human MGAT4B Polyclonal Antibody | anti-MGAT4B antibody

MGAT4B (Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase B, N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase IVb, GlcNAc-T IVb, GnT-IVb, N-acetylglucosaminyltransferase IVb, UDP-N-acetylglucosamine: alpha-1,3-D

Cat. Num. AAA6006809

• Gene Names: MGAT4B; GNT-IV; GNT-IVB
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: MGAT4B; Polyclonal Antibody; MGAT4B (Alpha-1; 3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase B; N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase IVb; GlcNAc-T IVb; GnT-IVb; N-acetylglucosaminyltransferase IVb; UDP-N-acetylglucosamine: alpha-1; 3-D; Anti -MGAT4B (Alpha-1; anti-MGAT4B antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MGAT4B.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.
• Sequence: MSRVAGTRTDVNELLQRWTPRCVRWHTGGARRVALDRPLVTACLPPAGDVVDVYQREFLALRDRLHAAEQESLKRSKELNLVLDEIKRAVSERQALRDGDGNRTWGRLTEDPRLKPWNGSHRHVLHLPTVFHHLPHLLAKESSLQPAVRVGQGRTGVSVVMGIPSVRREVHSYLTDTLHSLISELSPQEKEDSVIVVLIAETDSQYTSAVTENIKALFPTEIHSGLLEVISPSPHFYPDFSRLRESFGDPKERVRWRTKQNLDYCFLMMYAQSKGIYYVQLEDDIVAKPNYLSTMKNFALQQPSEDWMILEFSQLGFIGKMFKSLDLSLIVEFILMFYRDKPIDWLLDHILWVKVCNPEKDAKHCDRQKANLRIRFKPSLFQHVGTHSSLAGKIQKLKDKDFGKQALRKEHVNPPAEVSTSLKTYQHFTLEKAYLREDFFWAFTPAAGDFIRFRFFQPLRLERFFFRSGNIEHPEDKLFNTSVEVLPFDNPQSDKEALQEGRTATLRYPRSPDGYLQIGSFYKGVAEGEVDPAFGPLEALRLSIQTDSPVWVILSEIFLKKAD

• Applicable Applications for anti-MGAT4B antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.
• Immunogen: Full length human MGAT4B, aa1-563 (NP_463459.1).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of MGAT4B expression in transfected 293T cell line by MGAT4B polyclonal antibody. Lane 1: MGAT4B transfected lysate (61.93kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-MGAT4B antibody

Glycosyltransferase that participates in the transfer of N-acetylglucosamine (GlcNAc) to the core mannose residues of N-linked glycans. Catalyzes the formation of the GlcNAcbeta1-4 branch on the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans. Essential for the production of tri- and tetra-antennary N-linked sugar chains. Has lower affinities for donors or acceptors than MGAT4A, suggesting that, under physiological conditions, it is not the main contributor in N-glycan biosynthesis.

Product Categories/Family for anti-MGAT4B antibody

Antibodies; Abs to Carbohydrates; Glycoproteins

NCBI and Uniprot Product Information

• NCBI GI #: 62914020
• NCBI GeneID: 11282
• UniProt Accession #: Q9UQ53; Q86TF1; Q96GH4; Q9NSK6; A8MPR0
• Molecular Weight: 63,198 Da
• NCBI Official Full Name: MGAT4B protein, partial
• NCBI Official Synonym Full Names: mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme B
• NCBI Official Symbol: MGAT4B
• NCBI Official Synonym Symbols: GNT-IV; GNT-IVB
• NCBI Protein Information: alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase B; glcNAc-T IVb; N-acetylglucosaminyltransferase IVb; alpha-1,3-mannosyl-glycoprotein beta-1,4-N-acetylglucosaminyltransferase; N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase IVb; mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isoenzyme B; UDP-N-acetylglucosamine: alpha-1,3-D-mannoside beta-1,4-N-acetylglucosaminyltransferase IV; UDP-N-acetylglucosamine: alpha-1,3-D-mannoside beta-1,4-N-acetylglucosaminyltransferase IVb
• UniProt Protein Name: Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase B
• Protein Family: Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase
• UniProt Gene Name: MGAT4B
• UniProt Synonym Gene Names: GlcNAc-T IVb; GnT-IVb
• UniProt Entry Name: MGT4B_HUMAN

NCBI Description

This gene encodes a key glycosyltransferase that regulates the formation of tri- and multiantennary branching structures in the Golgi apparatus. The encoded protein, in addition to the related isoenzyme A, catalyzes the transfer of N-acetylglucosamine (GlcNAc) from UDP-GlcNAc in a beta-1,4 linkage to the Man-alpha-1,3-Man-beta-1,4-GlcNAc arm of R-Man-alpha-1,6(GlcNAc-beta-1,2-Man-alpha-1,3)Man-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1-Asn. The encoded protein may play a role in regulating the availability of serum glycoproteins, oncogenesis, and differentiation. [provided by RefSeq, Jul 2008]

Uniprot Description

MGAT4B iso3: Glycosyltransferase that participates in the transfer of N-acetylglucosamine (GlcNAc) to the core mannose residues of N- linked glycans. Catalyzes the formation of the GlcNAcbeta1-4 branch on the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans. Essential for the production of tri- and tetra-antennary N-linked sugar chains. Has lower affinities for donors or acceptors than MGAT4A, suggesting that, under physiological conditions, it is not the main contributor in N- glycan biosynthesis. Belongs to the glycosyltransferase 54 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Glycan Metabolism - N-glycan biosynthesis; EC 2.4.1.145; Transferase

Chromosomal Location of Human Ortholog: 5q35

Cellular Component: Golgi membrane; integral to membrane

Molecular Function: alpha-1,3-mannosylglycoprotein 4-beta-N-acetylglucosaminyltransferase activity; metal ion binding

Biological Process: cellular protein metabolic process; protein amino acid N-linked glycosylation via asparagine; N-glycan processing; post-translational protein modification