Mouse Thrombospondin 1 (THBS1) ELISA Kit | THBS1 elisa kit

Mouse Thrombospondin 1 (THBS1) High Sensitive ELISA Kit

Cat. Num. AAA2708021

• Gene Names: Thbs1; TSP1; TSP-1; tbsp1; Thbs-1
• Reactivity: Mouse
• Applications: ELISA
• Synonyms: Thrombospondin 1 (THBS1); Mouse Thrombospondin 1 (THBS1) High Sensitive ELISA Kit; Thrombospondin 1; TSP1; Thrombospondin-1p180; THBS1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of High Sensitive Thrombospondin 1 (THBS1).; No significant cross-reactivity or interference between High Sensitive Thrombospondin 1 (THBS1) and analogues was observed.
• Sequence Length: 1170

• Applicable Applications for THBS1 elisa kit: ELISA (EIA)
• Assay Type: Double-antibody Sandwich
• Samples: Plasma, tissue homogenates and other biological fluids
• Detection Range: 62.5-4,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 26.7pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Thrombospondin 1 (THBS1) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Thrombospondin 1 (THBS1) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Thrombospondin 1 (THBS1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Thrombospondin 1 (THBS1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Thrombospondin 1 (THBS1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of High Sensitive Thrombospondin 1 (THBS1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 549134
• NCBI GeneID: 21825
• UniProt Accession #: P35441
• NCBI Official Full Name: Thrombospondin-1
• NCBI Official Synonym Full Names: thrombospondin 1
• NCBI Official Symbol: Thbs1
• NCBI Official Synonym Symbols: TSP1; TSP-1; tbsp1; Thbs-1
• NCBI Protein Information: thrombospondin-1
• UniProt Protein Name: Thrombospondin-1
• Protein Family: Thrombospondin
• UniProt Gene Name: Thbs1
• UniProt Synonym Gene Names: Tsp1
• UniProt Entry Name: TSP1_MOUSE

NCBI Description

The protein encoded by this gene is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1. This protein has been shown to play roles in platelet aggregation, angiogenesis, and tumorigenesis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]

Uniprot Description

THBS1: Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Binds heparin. May play a role in dentinogenesis and/or maintenance of dentin and dental pulp. Ligand for CD36 mediating antiangiogenic properties. Belongs to the thrombospondin family.

Protein type: Motility/polarity/chemotaxis; Inhibitor

Cellular Component: cell surface; cytoplasm; endoplasmic reticulum; external side of plasma membrane; extracellular matrix; extracellular region; extracellular space; fibrinogen complex; sarcoplasmic reticulum; secretory granule

Molecular Function: calcium ion binding; extracellular matrix binding; fibroblast growth factor binding; fibronectin binding; heparin binding; integrin binding; laminin binding; low-density lipoprotein binding; phosphatidylserine binding; protein binding; transforming growth factor beta binding

Biological Process: activation of MAPK activity; behavioral response to pain; blood vessel morphogenesis; cell adhesion; cell cycle arrest; cell migration; engulfment of apoptotic cell; inflammatory response; negative regulation of angiogenesis; negative regulation of antigen processing and presentation of peptide or polysaccharide antigen via MHC class II; negative regulation of apoptosis; negative regulation of blood vessel endothelial cell migration; negative regulation of caspase activity; negative regulation of cell-matrix adhesion; negative regulation of dendritic cell antigen processing and presentation; negative regulation of endothelial cell proliferation; negative regulation of fibrinolysis; negative regulation of fibroblast growth factor receptor signaling pathway; negative regulation of interleukin-12 production; peptide cross-linking; positive regulation of angiogenesis; positive regulation of blood coagulation; positive regulation of blood vessel endothelial cell migration; positive regulation of cell migration; positive regulation of chemotaxis; positive regulation of macrophage activation; positive regulation of phosphorylation; positive regulation of protein kinase B signaling cascade; positive regulation of transforming growth factor beta receptor signaling pathway; positive regulation of translation; positive regulation of tumor necrosis factor biosynthetic process; regulation of cGMP metabolic process; response to calcium ion; response to glucose stimulus; response to magnesium ion; response to mechanical stimulus; response to unfolded protein; sprouting angiogenesis