Western Blot (WB)
(MGAT2 Antibody (P423) western blot analysis in MCF-7?HepG2 cell line lysates (35ug/lane).This demonstrates the MGAT2 antibody detected the MGAT2 protein (arrow).)
Rabbit anti-Human MGAT2 Polyclonal Antibody | anti-MGAT2 antibody
MGAT2, CT (Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase, Mannoside Acetylglucosaminyltransferase 2, N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase II, Beta-1,2-N-Acetylglucosaminyltransferase II, GlcNAc-T
Reactivity
Human
Applications
Immunohistochemistry, ELISA
Purity
Purified by Protein G Affinity Chromatography.
Synonyms
MGAT2; Polyclonal Antibody; CT (Alpha-1; 6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase; Mannoside Acetylglucosaminyltransferase 2; N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase II; Beta-1; 2-N-Acetylglucosaminyltransferase II; GlcNAc-T; anti-MGAT2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human MGAT2.
Purity/Purification
Purified by Protein G Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-MGAT2 antibody
Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
IHC: 1:50-1:100
ELISA: 1:1,000
Applications are based on unconjugated antibody.
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the C-terminal region of human MGAT2 (KLH).
Conjugate
AP
Note
Preservative Free
Preparation and Storage
May be stored at 4 degree C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(MGAT2 Antibody (P423) western blot analysis in MCF-7?HepG2 cell line lysates (35ug/lane).This demonstrates the MGAT2 antibody detected the MGAT2 protein (arrow).)
Western Blot (WB)
(MGAT2 Antibody (P423) western blot analysis in MCF-7?HepG2 cell line lysates (35ug/lane).This demonstrates the MGAT2 antibody detected the MGAT2 protein (arrow).)
Flow Cytometry (FC/FACS)
(MGAT2 Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(MGAT2 Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-MGAT2 antibody
References
1. Yen, C.L., et al., J. Biol. Chem. 278(20):18532-18537 (2003). 2. Chen, S.H., et al., Glycoconj. J. 15(3):301-308 (1998). 3. Tan, J., et al., Am. J. Hum. Genet. 59(4):810-817 (1996). 4. Tan, J., et al., Eur. J. Biochem. 231(2):317-328 (1995). 5. D'Agostaro, G.A., et al., J. Biol. Chem. 270(25):15211-15221 (1995).
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Product Notes
The MGAT2 (Catalog #AAA6487216) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MGAT2, CT (Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase, Mannoside Acetylglucosaminyltransferase 2, N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase II, Beta-1,2-N-Acetylglucosaminyltransferase II, GlcNAc-T reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MGAT2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA). IHC: 1:50-1:100 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the MGAT2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MGAT2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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