Rabbit anti-Human MFI2 Polyclonal Antibody | anti-MFI2 antibody

MFI2, CT (MFI2, MAP97, Melanotransferrin, Melanoma-associated antigen p97, CD228) (PE)

Cat. Num. AAA6320524

• Gene Names: MFI2; MTF1; CD228; MAP97
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MFI2; Polyclonal Antibody; CT (MFI2; MAP97; Melanotransferrin; Melanoma-associated antigen p97; CD228) (PE); CD228; anti-MFI2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MFI2.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-MFI2 antibody: Western Blot (WB), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 627-655 in the C-terminal region of human MFI2, UniProt Accession #NP_005920.2; P08582. Species sequence homology: mouse and rabbit
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in MDA-MB231 cell line lysates (35ug/lane) usingMBS6001624. This demonstrates that MBS6001624 detected the MFI2 protein (arrow).)

Related Product Information for anti-MFI2 antibody

The protein encoded by this gene is a cell-surface glycoprotein found on melanoma cells. The protein shares sequence similarity and iron-binding properties with members of the transferrin superfamily. The importance of the iron binding function has not yet been identified. This gene resides in the same region of chromosome 3 as members of the transferrin superfamily. Alternative splicing results in two transcript variants. [provided by RefSeq].

Product Categories/Family for anti-MFI2 antibody

Antibodies; CD Markers

References

1. Wang, M., et al. J. Biol. Chem. 284(48):33377-33383(2009). 2. Farnaud, S., et al. Int. J. Biochem. Cell Biol. 40(12):2739-2745(2008). 3. Suryo Rahmanto, Y., et al. Carcinogenesis 28(10):2172-2183(2007). 4. Suryo Rahmanto, Y., et al. Oncogene 26(42):6113-6124(2007). 5. Michaud-Levesque, J., et al. Biol. Chem. 388(7):747-754(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 134244281
• NCBI GeneID: 4241
• NCBI Accession #: NP_005920.2
• NCBI GenBank Nucleotide #: NM_005929.5
• UniProt Accession #: P08582; Q9BQE2
• Molecular Weight: 80,215 Da
• NCBI Official Full Name: melanotransferrin isoform 1
• NCBI Official Synonym Full Names: antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and 96.5
• NCBI Official Symbol: MFI2
• NCBI Official Synonym Symbols: MTF1; CD228; MAP97
• NCBI Protein Information: melanotransferrin; membrane-bound transferrin-like protein; melanoma-associated antigen p97, isoform 2
• UniProt Protein Name: Melanotransferrin
• UniProt Gene Name: MFI2
• UniProt Synonym Gene Names: MAP97
• UniProt Entry Name: TRFM_HUMAN

NCBI Description

The protein encoded by this gene is a cell-surface glycoprotein found on melanoma cells. The protein shares sequence similarity and iron-binding properties with members of the transferrin superfamily. The importance of the iron binding function has not yet been identified. This gene resides in the same region of chromosome 3 as members of the transferrin superfamily. Alternative splicing results in two transcript variants. [provided by RefSeq, Jul 2008]

Uniprot Description

MFI2: Involved in iron cellular uptake. Seems to be internalized and then recycled back to the cell membrane. Binds a single atom of iron per subunit. Could also bind zinc. Belongs to the transferrin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 3q28-q29

Cellular Component: cell surface; anchored to plasma membrane; integral to plasma membrane

Molecular Function: protein binding; ferric iron binding; iron ion binding

Biological Process: cellular iron ion homeostasis; regulation of cell growth; regulation of cell proliferation