Rabbit anti-Human MFI2 Polyclonal Antibody | anti-MFI2 antibody

MFI2, CT (MFI2, MAP97, Melanotransferrin, Melanoma-associated antigen p97, CD228) (Azide free) (HRP)

Cat. Num. AAA6320518

• Gene Names: MFI2; MTF1; CD228; MAP97
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MFI2; Polyclonal Antibody; CT (MFI2; MAP97; Melanotransferrin; Melanoma-associated antigen p97; CD228) (Azide free) (HRP); CD228; anti-MFI2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MFI2.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-MFI2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 627-655 in the C-terminal region of human MFI2, UniProt Accession #NP_005920.2; P08582. Species sequence homology: mouse and rabbit
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in MDA-MB231 cell line lysates (35ug/lane) usingMBS6001624. This demonstrates that MBS6001624 detected the MFI2 protein (arrow).)

Related Product Information for anti-MFI2 antibody

The protein encoded by this gene is a cell-surface glycoprotein found on melanoma cells. The protein shares sequence similarity and iron-binding properties with members of the transferrin superfamily. The importance of the iron binding function has not yet been identified. This gene resides in the same region of chromosome 3 as members of the transferrin superfamily. Alternative splicing results in two transcript variants. [provided by RefSeq].

Product Categories/Family for anti-MFI2 antibody

Antibodies; CD Markers

References

1. Wang, M., et al. J. Biol. Chem. 284(48):33377-33383(2009). 2. Farnaud, S., et al. Int. J. Biochem. Cell Biol. 40(12):2739-2745(2008). 3. Suryo Rahmanto, Y., et al. Carcinogenesis 28(10):2172-2183(2007). 4. Suryo Rahmanto, Y., et al. Oncogene 26(42):6113-6124(2007). 5. Michaud-Levesque, J., et al. Biol. Chem. 388(7):747-754(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 134244281
• NCBI GeneID: 4241
• NCBI Accession #: NP_005920.2
• NCBI GenBank Nucleotide #: NM_005929.5
• UniProt Accession #: P08582; Q9BQE2
• Molecular Weight: 80,215 Da
• NCBI Official Full Name: melanotransferrin isoform 1
• NCBI Official Synonym Full Names: antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and 96.5
• NCBI Official Symbol: MFI2
• NCBI Official Synonym Symbols: MTF1; CD228; MAP97
• NCBI Protein Information: melanotransferrin; membrane-bound transferrin-like protein; melanoma-associated antigen p97, isoform 2
• UniProt Protein Name: Melanotransferrin
• UniProt Gene Name: MFI2
• UniProt Synonym Gene Names: MAP97
• UniProt Entry Name: TRFM_HUMAN

NCBI Description

The protein encoded by this gene is a cell-surface glycoprotein found on melanoma cells. The protein shares sequence similarity and iron-binding properties with members of the transferrin superfamily. The importance of the iron binding function has not yet been identified. This gene resides in the same region of chromosome 3 as members of the transferrin superfamily. Alternative splicing results in two transcript variants. [provided by RefSeq, Jul 2008]

Uniprot Description

MFI2: Involved in iron cellular uptake. Seems to be internalized and then recycled back to the cell membrane. Binds a single atom of iron per subunit. Could also bind zinc. Belongs to the transferrin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 3q28-q29

Cellular Component: cell surface; anchored to plasma membrane; integral to plasma membrane

Molecular Function: protein binding; ferric iron binding; iron ion binding

Biological Process: cellular iron ion homeostasis; regulation of cell growth; regulation of cell proliferation