Rabbit anti-Human MEX3C Polyclonal Antibody | anti-MEX3C antibody

MEX3C, CT (MEX3C, RKHD2, RNF194, RNA-binding protein MEX3C, RING finger and KH domain-containing protein 2, RING finger protein 194) (Biotin)

Cat. Num. AAA6320472

• Gene Names: MEX3C; RKHD2; BM-013; MEX-3C; RNF194
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MEX3C; Polyclonal Antibody; CT (MEX3C; RKHD2; RNF194; RNA-binding protein MEX3C; RING finger and KH domain-containing protein 2; RING finger protein 194) (Biotin); RING finger protein 194; anti-MEX3C antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-MEX3C antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: MEX3C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 536-564 amino acids from the C-terminal region of human MEX3C.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(MEX3C Antibody (C-term) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the MEX3C antibody detected the MEX3C protein (arrow).)

Related Product Information for anti-MEX3C antibody

This gene encodes a member of a family of proteins with two K homology (KH) RNA-binding domains and a C-terminal RING-finger domain. The protein interacts with mRNA via the KH domains, and the protein shuttles between the nucleus and cytoplasm. Polymorphisms in this gene may contribute to hypertension.

Product Categories/Family for anti-MEX3C antibody

Antibodies; Transcription Factors; Zinc (Ring) Finger

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Buchet-Poyau, K., et al. Nucleic Acids Res. 35(4):1289-1300(2007). Guzman, B., et al. Hypertension 48(5):883-891(2006). Nusbaum, C., et al. Nature 437(7058):551-555(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 148229134
• NCBI GeneID: 51320
• NCBI Accession #: NP_057710.3
• NCBI GenBank Nucleotide #: NM_016626.4
• UniProt Accession #: Q5U5Q3; Q9NZE3; A1L022
• Molecular Weight: 69,366 Da
• NCBI Official Full Name: RNA-binding E3 ubiquitin-protein ligase MEX3C
• NCBI Official Synonym Full Names: mex-3 RNA binding family member C
• NCBI Official Symbol: MEX3C
• NCBI Official Synonym Symbols: RKHD2; BM-013; MEX-3C; RNF194
• NCBI Protein Information: RNA-binding E3 ubiquitin-protein ligase MEX3C
• UniProt Protein Name: RNA-binding E3 ubiquitin-protein ligase MEX3C
• Protein Family: RNA-binding E3 ubiquitin-protein ligase
• UniProt Gene Name: MEX3C
• UniProt Synonym Gene Names: RKHD2; RNF194
• UniProt Entry Name: MEX3C_HUMAN

NCBI Description

This gene encodes a member of a family of proteins with two K homology (KH) RNA-binding domains and a C-terminal RING-finger domain. The protein interacts with mRNA via the KH domains, and the protein shuttles between the nucleus and cytoplasm. Polymorphisms in this gene may contribute to hypertension. [provided by RefSeq, Oct 2009]

Uniprot Description

RKHD2: E3 ubiquitin ligase responsible for the post- transcriptional regulation of common HLA-A allotypes. Binds to the 3' UTR of HLA-A2 mRNA, and regulates its levels by promoting mRNA decay. RNA binding is sufficient to prevent translation, but ubiquitin ligase activity is required for mRNA degradation. Genetic variations in MEX3C may be associated with susceptibility to essential hypertension.

Protein type: RNA-binding; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 18q21.2

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; zinc ion binding; ligase activity

Biological Process: regulation of fat cell differentiation; metabolic process

Disease: Hypertension, Essential, Susceptibility To, 8