Rabbit METTL3 Polyclonal Antibody | anti-METTL3 antibody

METTL3 Rabbit pAb

Cat. Num. AAA4757664

• Gene Names: METTL3; M6A; IME4; Spo8; MT-A70
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Affinity purified
• Synonyms: METTL3; Polyclonal Antibody; METTL3 Rabbit pAb; N6-adenosine-methyltransferase catalytic subunit; methyltransferase like 3; M6A; IME4; Spo8; MT-A70; hMETTL3; anti-METTL3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Widely expressed at low level. Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes.
• Purity/Purification: Affinity purified
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

• Applicable Applications for anti-METTL3 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IHC1: 1:50-1:200; IF: 1:50-1:200; IP: 1:20-1:50
• Immunogen: Recombinant protein of human METTL3 (NP_062826.2).
• Conjugation: Unconjugated
• Molecular Function: The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues at the N6 position of some RNAs and regulates various processes such as the circadian clock, differentiation of embryonic and haematopoietic stem cells, cortical neurogenesis, response to DNA damage, differentiation of T-cells and primary miRNA processing (PubMed:22575960, PubMed:24284625, PubMed:25719671, PubMed:25799998, PubMed:26321680, PubMed:26593424, PubMed:27627798, PubMed:27373337, PubMed:27281194, PubMed:28297716, PubMed:9409616). In the heterodimer formed with METTL14, METTL3 constitutes the catalytic core (PubMed:27627798, PubMed:27373337, PubMed:27281194). N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in mRNA stability, processing, translation efficiency and editing (PubMed:22575960, PubMed:24284625, PubMed:25719671, PubMed:25799998, PubMed:26321680, PubMed:26593424, PubMed:28297716, PubMed:9409616). M6A acts as a key regulator of mRNA stability: methylation is completed upon the release of mRNA into the nucleoplasm and promotes mRNA destabilization and degradation (PubMed:28637692). In embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization, promoting differentiation of ESCs. M6A regulates the length of the circadian clock: acts as an early pace-setter in the circadian loop by putting mRNA production on a fast-track for facilitating nuclear processing, thereby providing an early point of control in setting the dynamics of the feedback loop. M6A regulates spermatogonial differentiation and meiosis and is essential for male fertility and spermatogenesis. Involved in the response to DNA damage: in response to ultraviolet irradiation, METTL3 rapidly catalyzes the formation of m6A on poly(A) transcripts at DNA damage sites, leading to the recruitment of POLK to DNA damage sites (PubMed:28297716). M6A is also required for T-cell homeostasis and differentiation: m6A methylation of transcripts of SOCS family members (SOCS1, SOCS3 and CISH) in naive T-cells promotes mRNA destabilization and degradation, promoting T-cell differentiation. M6A also takes place in other RNA molecules, such as primary miRNA (pri-miRNAs) (PubMed:25799998). M6A also regulates cortical neurogenesis: m6A methylation of transcripts related to transcription factors, neural stem cells, the cell cycle and neuronal differentiation during brain development promotes their destabilization and decay, promoting differentiation of radial glial cells. METTL3 mediates methylation of pri-miRNAs, marking them for recognition and processing by DGCR8 (PubMed:25799998). Acts as a positive regulator of mRNA translation independently of the methyltransferase activity: promotes translation by interacting with the translation initiation machinery in the cytoplasm (PubMed:27117702). Its overexpression in a number of cancer cells suggests that it may participate to cancer cell proliferation by promoting mRNA translation (PubMed:27117702).
• Modification: Unmodified
• Cell Position: Cytoplasm, Nucleus
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot - METTL3 Polyclonal Antibody.Western blot analysis of extracts of various cell lines, using METTL3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 1s.)

Immunofluorescence (IF)

(Immunofluorescence - METTL3 Polyclonal Antibody. Immunofluorescence analysis of C6 cells using METTL3 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

Immunofluorescence (IF)

(Immunofluorescence - METTL3 Polyclonal Antibody. Immunofluorescence analysis of HeLa cells using METTL3 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

Immunofluorescence (IF)

(Immunofluorescence - METTL3 Polyclonal Antibody. Immunofluorescence analysis of NIH/3T3 cells using METTL3 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

Immunofluorescence (IF)

(Immunofluorescence - METTL3 Polyclonal Antibody Confocal immunofluorescence analysis of Hela cells using METTL3 Polyclonal Antibody at dilution of 1:200. Blue: DAPI for nuclear staining.)

Immunofluorescence (IF)

(Immunofluorescence - METTL3 Polyclonal Antibody Confocal immunofluorescence analysis of U-2 OS cells using METTL3 Polyclonal Antibody at dilution of 1:200. Blue: DAPI for nuclear staining.)

Immunoprecipitation (IP)

(Immunoprecipitation - METTL3 Polyclonal Antibody Immunoprecipitation analysis of 150ug extracts of 293T cells using 3ug METTL3 antibody . Western blot was performed from the immunoprecipitate using METTL3 antibody at a dilition of 1:1000.)

Related Product Information for anti-METTL3 antibody

This gene encodes the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine. [provided by RefSeq, Jul 2008]

Product Categories/Family for anti-METTL3 antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 21361827
• NCBI GeneID: 56339
• NCBI Accession #: NP_062826
• NCBI GenBank Nucleotide #: NM_019852
• UniProt Accession #: Q86U44; O14736; Q86V05; Q9HB32
• Molecular Weight: 25,479 Da
• NCBI Official Full Name: N6-adenosine-methyltransferase 70 kDa subunit
• NCBI Official Synonym Full Names: methyltransferase like 3
• NCBI Official Symbol: METTL3
• NCBI Official Synonym Symbols: M6A; IME4; Spo8; MT-A70
• NCBI Protein Information: N6-adenosine-methyltransferase 70 kDa subunit; adoMet-binding subunit of the human mRNA (N6-adenosine)-methyltransferase; mRNA (2'-O-methyladenosine-N(6)-)-methyltransferase; mRNA m(6)A methyltransferase; methyltransferase-like protein 3
• UniProt Protein Name: N6-adenosine-methyltransferase 70 kDa subunit
• Protein Family: N6-adenosine-methyltransferase
• UniProt Gene Name: METTL3
• UniProt Synonym Gene Names: MTA70; MT-A70
• UniProt Entry Name: MTA70_HUMAN