Western Blot (WB)
(Western blot analysis of METTL3 expression in (1) 293 cell lysate; (2) RAW 264.7 cell lysate.)
Rabbit METTL3 Polyclonal Antibody | anti-METTL3 antibody
METTL3 Rabbit pAb
Gene Names
METTL3; M6A; IME4; Spo8; MT-A70
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Flow Cytometry, Functional Assay
Purity
Affinity Chromatography
Synonyms
METTL3; Polyclonal Antibody; METTL3 Rabbit pAb; IME4; M6A; Methyltransferase like protein 3; MTA70; N6 adenosine methyltransferase 70 kDa subunit; anti-METTL3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Widely expressed at low level. Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes.
METTL3 Antibody detects endogenous levels of total METTL3
METTL3 Antibody detects endogenous levels of total METTL3
Purity/Purification
Affinity Chromatography
Form/Format
Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applicable Applications for anti-METTL3 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
IP: 1:50
FC/FACS: 1:50
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
IP: 1:50
FC/FACS: 1:50
Immunogen
A synthesized peptide derived from human METTL3
Conjugation
Unconjugated
Modification
Unmodified
Cell Position
Cytoplasm, Nucleus
Preparation and Storage
Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.
Western Blot (WB)
(Western blot analysis of METTL3 expression in (1) 293 cell lysate; (2) RAW 264.7 cell lysate.)
Western Blot (WB)
(Western blot analysis of METTL3 expression in (1) 293 cell lysate; (2) RAW 264.7 cell lysate.)
Related Product Information for anti-METTL3 antibody
N6-methyltransferase that methylates adenosine residues of some mRNAs. N6-methyladenosine (m6A), which is present at internal sites of some mRNAs, may play a role in the efficiency of mRNA splicing, transport or translation.
Product Categories/Family for anti-METTL3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25,479 Da
NCBI Official Full Name
N6-adenosine-methyltransferase 70 kDa subunit
NCBI Official Synonym Full Names
methyltransferase like 3
NCBI Official Symbol
METTL3
NCBI Official Synonym Symbols
M6A; IME4; Spo8; MT-A70
NCBI Protein Information
N6-adenosine-methyltransferase 70 kDa subunit; adoMet-binding subunit of the human mRNA (N6-adenosine)-methyltransferase; mRNA (2'-O-methyladenosine-N(6)-)-methyltransferase; mRNA m(6)A methyltransferase; methyltransferase-like protein 3
UniProt Protein Name
N6-adenosine-methyltransferase 70 kDa subunit
Protein Family
UniProt Gene Name
METTL3
UniProt Synonym Gene Names
MTA70; MT-A70
UniProt Entry Name
MTA70_HUMAN
Similar Products
Product Notes
The METTL3 mettl3 (Catalog #AAA4755617) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The METTL3 Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's METTL3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). WB: 1:500-1:2000 IHC: 1:50-1:200 ICC/IF: 1:50-1:200 IP: 1:50 FC/FACS: 1:50. Researchers should empirically determine the suitability of the METTL3 mettl3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "METTL3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
