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Typical Testing Data/Standard Curve (for reference only)

Mouse thyrotropin-releasing hormone ELISA Kit | TRH elisa kit

Mouse thyrotropin-releasing hormone, TRH ELISA Kit

Gene Names
Trh; Trf; Pro-TRH
Reactivity
Mouse
Synonyms
thyrotropin-releasing hormone; Mouse thyrotropin-releasing hormone; TRH ELISA Kit; TRH ELISA KIT; MGC125964; MGC125965; ; TRH elisa kit
Ordering
For Research Use Only!
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse TRH. No significant cross-reactivity or interference between mouse TRH and analogues was observed.
Sequence Length
256
Samples
Serum
Assay Type
Quantitative Sandwich
Detection Range
2.5 pg/ml-160 pg/ml
Sensitivity
< 0.625 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for TRH elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TRH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TRH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TRH is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRH bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
29,195 Da
NCBI Official Full Name
thyrotropin releasing hormone preproprotein
NCBI Official Synonym Full Names
thyrotropin releasing hormone
NCBI Official Symbol
Trh
NCBI Official Synonym Symbols
Trf; Pro-TRH
NCBI Protein Information
thyrotropin releasing hormone; protirelin; prothyroliberin; TSH-releasing factor; thyrotropin-releasing factor; pro-thyrotropin-releasing hormone
UniProt Protein Name
Pro-thyrotropin-releasing hormone
UniProt Gene Name
Trh
UniProt Synonym Gene Names
Pro-TRH; TRH; TRF
UniProt Entry Name
TRH_MOUSE

NCBI Description

This gene encodes a member of the thyrotropin-releasing hormone family. Cleavage of the encoded proprotein releases mature thyrotropin-releasing hormone, which is a tripeptide hypothalamic regulatory hormone. The mouse proprotein contains five thyrotropin-releasing hormone tripeptides. Thyrotropin-releasing hormone is involved in the regulation and release of thyroid-stimulating hormone, as well as prolactin. Disruption of this gene results in hypothyroidism, elevated thyroid-stimulating hormone levels, and hyperglycemia. [provided by RefSeq, Apr 2013]

Uniprot Description

Function: Functions as a regulator of the biosynthesis of TSH in the anterior pituitary gland and as a neurotransmitter/ neuromodulator in the central and peripheral nervous systems.

Research Articles on TRH

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Product Notes

The Mouse TRH trh (Catalog #AAA700675) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA700675 ELISA Kit recognizes Mouse TRH. It is sometimes possible for the material contained within the vial of "thyrotropin-releasing hormone, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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