Immunocytochemistry (ICC)
(Immunocytochemistry Analysis: Representative lot data. Confocal fluorescent analysis of NIH/3T3 and HeLa cells using MBS628047 (Red). Actin filaments have been labeled with AlexaFluor®488 -Phalloidin (Green). The nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.)
Rabbit anti-Human, Mouse Metadherin Polyclonal Antibody | anti-MTDH antibody
Metadherin (MTDH, 3D3, 3D3/LYRIC, AEG1, AEG-1, Astrocyte elevated gene-1 protein, LYRIC, LYRIC/3D3, Lysine-rich CEACAM1 co-isolated protein, Metastasis adhesion protein, Protein LYRIC)
Gene Names
MTDH; 3D3; AEG1; AEG-1; LYRIC; LYRIC/3D3
Reactivity
Human, Mouse
Applications
Western Blot, Immunocytochemistry
Purity
Affinity PurifiedPurified by affinity chromatography.
Synonyms
Metadherin; Polyclonal Antibody; Metadherin (MTDH; 3D3; 3D3/LYRIC; AEG1; AEG-1; Astrocyte elevated gene-1 protein; LYRIC; LYRIC/3D3; Lysine-rich CEACAM1 co-isolated protein; Metastasis adhesion protein; Protein LYRIC); Anti -Metadherin (MTDH; anti-MTDH antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes mouse Metadherin. Species Crossreactivity: human.
Purity/Purification
Affinity Purified
Purified by affinity chromatography.
Purified by affinity chromatography.
Form/Format
Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Applicable Applications for anti-MTDH antibody
Western Blot (WB), Immunocytochemistry (ICC)
Application Notes
Suitable for use in Western Blot and Immunocytochemistry.
Dilution: Western Blot: 0.25ug/ml. Detected metadherin in 10ug of HEK293, HepG2 and Jurkat cell lysates.
Immunocytochemistry: 1:500. Detected metadherin in NIH/3T3 and HeLa cells.
Dilution: Western Blot: 0.25ug/ml. Detected metadherin in 10ug of HEK293, HepG2 and Jurkat cell lysates.
Immunocytochemistry: 1:500. Detected metadherin in NIH/3T3 and HeLa cells.
Immunogen
Recombinant proteins corresponding at or around the lung homing domain of mouse Metadherin (GST-tagged).
Positive Control
HeLa cell lysate
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunocytochemistry (ICC)
(Immunocytochemistry Analysis: Representative lot data. Confocal fluorescent analysis of NIH/3T3 and HeLa cells using MBS628047 (Red). Actin filaments have been labeled with AlexaFluor®488 -Phalloidin (Green). The nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.)
Immunocytochemistry (ICC)
(Immunocytochemistry Analysis: Representative lot data. Confocal fluorescent analysis of NIH/3T3 and HeLa cells using MBS628047 (Red). Actin filaments have been labeled with AlexaFluor®488 -Phalloidin (Green). The nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.)
Western Blot (WB)
(Western Blott Analysis: Representative lot data. HeLa cell lysate was probed with MBS628047 (0.25ug/mL). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and chemiluminescence detection system. Arrow indicates Metadherin (~75kD).)
Western Blot (WB)
(Western Blott Analysis: Representative lot data. HeLa cell lysate was probed with MBS628047 (0.25ug/mL). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and chemiluminescence detection system. Arrow indicates Metadherin (~75kD).)
Related Product Information for anti-MTDH antibody
Metadherin is a type 2 transmembrane protein who's overexpression was first described in breast cancers. It plays an important role in the metastasis of breast cancers into the lungs as a secondary site of development. Antibodies reactive to the lung-homing domain of metadherin and siRNA-mediated knockdown of metadherin expression in breast cancer cells inhibited experimental lung metastasis, indicating that tumor cell metadherin mediates localization at the metastatic site (Brown, 2009).
Product Categories/Family for anti-MTDH antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
63,837 Da
NCBI Official Full Name
Metadherin
NCBI Official Synonym Full Names
metadherin
NCBI Official Symbol
MTDH
NCBI Official Synonym Symbols
3D3; AEG1; AEG-1; LYRIC; LYRIC/3D3
NCBI Protein Information
protein LYRIC; 3D3/LYRIC; OTTHUMP00000227231; OTTHUMP00000227366; metastasis adhesion protein; astrocyte elevated gene-1 protein; lysine-rich CEACAM1 co-isolated protein
UniProt Protein Name
Protein LYRIC
Protein Family
UniProt Gene Name
MTDH
UniProt Synonym Gene Names
AEG1; LYRIC
UniProt Entry Name
LYRIC_HUMAN
Uniprot Description
Lyric: a protein of unknown function.
Protein type: Nucleolus; Membrane protein, integral; Cell adhesion
Chromosomal Location of Human Ortholog: 8q22.1
Cellular Component: nuclear body; intercellular canaliculus; endoplasmic reticulum membrane; nuclear membrane; tight junction; perinuclear region of cytoplasm; endoplasmic reticulum; cytoplasm; apical plasma membrane; nucleolus; integral to membrane; nucleus
Molecular Function: NF-kappaB binding; protein binding; transcription coactivator activity; double-stranded RNA binding
Biological Process: positive regulation of protein kinase B signaling cascade; positive regulation of angiogenesis; positive regulation of I-kappaB kinase/NF-kappaB cascade; lipopolysaccharide-mediated signaling pathway; negative regulation of transcription from RNA polymerase II promoter; positive regulation of autophagy; activation of NF-kappaB transcription factor; negative regulation of apoptosis
Research Articles on MTDH
1. AEG-1 overexpression is associated with carcinogenesis and tumor progression in endometrial cancer.
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Product Notes
The MTDH mtdh (Catalog #AAA628047) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Metadherin (MTDH, 3D3, 3D3/LYRIC, AEG1, AEG-1, Astrocyte elevated gene-1 protein, LYRIC, LYRIC/3D3, Lysine-rich CEACAM1 co-isolated protein, Metastasis adhesion protein, Protein LYRIC) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Metadherin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC). Suitable for use in Western Blot and Immunocytochemistry. Dilution: Western Blot: 0.25ug/ml. Detected metadherin in 10ug of HEK293, HepG2 and Jurkat cell lysates. Immunocytochemistry: 1:500. Detected metadherin in NIH/3T3 and HeLa cells. Researchers should empirically determine the suitability of the MTDH mtdh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Metadherin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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