Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Immunocytochemistry (ICC) (Immunocytochemistry Analysis: Representative lot data. Confocal fluorescent analysis of NIH/3T3 and HeLa cells using MBS628047 (Red). Actin filaments have been labeled with AlexaFluor488-Phalloidin (Green). The nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.)

Rabbit anti-Mouse, Human Metadherin Polyclonal Antibody | anti-MTDH antibody

Metadherin (MTDH, 3D3, 3D3/LYRIC, AEG1, AEG-1, Astrocyte elevated gene-1 protein, LYRIC, LYRIC/3D3, Lysine-rich CEACAM1 co-isolated protein, Metastasis adhesion protein, Protein LYRIC) (AP)

Gene Names
Mtdh; 3D3; AEG-1; Lyric; AV353288; 3D3/Lyric; D8Bwg1112e; 2610103J23Rik
Reactivity
Mouse, Human
Applications
Immunocytochemistry, Western Blot
Purity
Purified by affinity chromatography.
Synonyms
Metadherin; Polyclonal Antibody; Metadherin (MTDH; 3D3; 3D3/LYRIC; AEG1; AEG-1; Astrocyte elevated gene-1 protein; LYRIC; LYRIC/3D3; Lysine-rich CEACAM1 co-isolated protein; Metastasis adhesion protein; Protein LYRIC) (AP); anti-MTDH antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse, Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes mouse Metadherin. Species Crossreactivity: human.
Purity/Purification
Purified by affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.
Applicable Applications for anti-MTDH antibody
Immunocytochemistry (ICC), Western Blot (WB)
Application Notes
ICC: 1:500. Detected metadherin in NIH/3T3 and HeLa cells.
WB: 0.25ug/ml. Detected metadherin in 10ug of HEK293, HepG2 and Jurkat cell lysates.
Applications are based on unconjugated antibody.
Immunogen
Recombinant proteins corresponding at or around the lung homing domain of mouse Metadherin (GST-tagged).
Conjugate
AP
Positive Control
HeLa Cell Lysate
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Immunocytochemistry (ICC)

(Immunocytochemistry Analysis: Representative lot data. Confocal fluorescent analysis of NIH/3T3 and HeLa cells using MBS628047 (Red). Actin filaments have been labeled with AlexaFluor488-Phalloidin (Green). The nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.)

Immunocytochemistry (ICC) (Immunocytochemistry Analysis: Representative lot data. Confocal fluorescent analysis of NIH/3T3 and HeLa cells using MBS628047 (Red). Actin filaments have been labeled with AlexaFluor488-Phalloidin (Green). The nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.)

Western Blot (WB)

(Western Blott Analysis: Representative lot data. HeLa cell lysate was probed with MBS628047 (0.25ug/mL). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and chemiluminescence detection system. Arrow indicates Metadherin (~75kD).)

Western Blot (WB) (Western Blott Analysis: Representative lot data. HeLa cell lysate was probed with MBS628047 (0.25ug/mL). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and chemiluminescence detection system. Arrow indicates Metadherin (~75kD).)
Product Categories/Family for anti-MTDH antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
63,846 Da
NCBI Official Full Name
protein LYRIC
NCBI Official Synonym Full Names
metadherin
NCBI Official Symbol
Mtdh
NCBI Official Synonym Symbols
3D3; AEG-1; Lyric; AV353288; 3D3/Lyric; D8Bwg1112e; 2610103J23Rik
NCBI Protein Information
protein LYRIC
UniProt Protein Name
Protein LYRIC
Protein Family
UniProt Gene Name
Mtdh
UniProt Synonym Gene Names
Lyric
UniProt Entry Name
LYRIC_MOUSE

Uniprot Description

Lyric: a protein of unknown function.

Protein type: Nucleolus; Cell adhesion; Membrane protein, integral

Cellular Component: nuclear body; intercellular canaliculus; endoplasmic reticulum membrane; tight junction; membrane; endoplasmic reticulum; perinuclear region of cytoplasm; cytoplasm; apical plasma membrane; integral to membrane; nucleolus; nucleus; cell junction

Molecular Function: NF-kappaB binding; protein binding; double-stranded RNA binding; transcription coactivator activity

Biological Process: positive regulation of protein kinase B signaling cascade; positive regulation of angiogenesis; positive regulation of I-kappaB kinase/NF-kappaB cascade; lipopolysaccharide-mediated signaling pathway; negative regulation of transcription from RNA polymerase II promoter; positive regulation of autophagy; negative regulation of apoptosis; activation of NF-kappaB transcription factor

Research Articles on MTDH

Similar Products

Product Notes

The MTDH mtdh (Catalog #AAA6487844) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Metadherin (MTDH, 3D3, 3D3/LYRIC, AEG1, AEG-1, Astrocyte elevated gene-1 protein, LYRIC, LYRIC/3D3, Lysine-rich CEACAM1 co-isolated protein, Metastasis adhesion protein, Protein LYRIC) (AP) reacts with Mouse, Human and may cross-react with other species as described in the data sheet. AAA Biotech's Metadherin can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Western Blot (WB). ICC: 1:500. Detected metadherin in NIH/3T3 and HeLa cells. WB: 0.25ug/ml. Detected metadherin in 10ug of HEK293, HepG2 and Jurkat cell lysates. Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the MTDH mtdh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Metadherin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.