Rabbit MET-Y1234 Polyclonal Antibody | anti-MET-Y1234 antibody

Phospho-MET-Y1234 Polyclonal Antibody

Cat. Num. AAA8527449

• Gene Names: MET; HGFR; AUTS9; RCCP2; c-Met; DFNB97
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity purification
• Synonyms: MET-Y1234; Polyclonal Antibody; Phospho-MET-Y1234 Polyclonal Antibody; HGFR; AUTS9; RCCP2; c-Met; anti-MET-Y1234 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purification
• Sequence Length: 1390

• Applicable Applications for anti-MET-Y1234 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A phospho specific peptide corresponding to residues surrounding Y1234 of human MET
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.

Related Product Information for anti-MET-Y1234 antibody

Met, a high affinity tyrosine kinase receptor for hepatocyte growth factor (HGF, also known as scatter factor) is a disulfide-linked heterodimer made of 45 kDa alpha-and 145 kDa beta-subunits (1, 2). The alpha-subunit and the amino-terminal region of the beta-subunit form the extracellular domain. The remainder of the beta-chain spans the plasma membrane and contains a cytoplasmic region with tyrosine kinase activity. Interaction of Met with HGF results in autophosphorylation at multiple tyrosines, which recruit several downstream signaling components, including Gab1, c-Cbl, and PI3 kinase (3). These fundamental events are important for all of the biological functions involving Met kinase activity. The addition of a phosphate at cytoplasmic Tyr1003 is essential for Met protein ubiquitination and degradation (4). Phosphorylation at Tyr1234/1235 in the Met kinase domain is critical for kinase activation. Phosphorylation at Tyr1349 in the Met cytoplasmic domain provides a direct binding site for Gab1 (5). Research studies have shown that altered Met levels and/or tyrosine kinase activities are found in several types of tumors, including renal, colon, and breast. Thus, investigators have concluded that Met is an attractive potential cancer target (6, 7).

References

1. Cooper, C.S. et al. (1984) Nature 311, 29-33. 2. Bottaro, D.P. et al. (1991) Science 251, 802-4. 3. Bardelli, A. et al. (1997) Oncogene 15, 3103-11. 4. Taher, T.E. et al. (2002) J Immunol 169, 3793-800. 5. Schaeper, U. et al. (2000) J Cell Biol 149, 1419-32. 6. Eder, J.P. et al. (2009) Clin Cancer Res 15, 2207-14. 7. Sattler, M. and Salgia, R. (2009) Update Cancer Ther 3, 109-118.

NCBI and Uniprot Product Information

• NCBI GI #: 42741655
• NCBI GeneID: 4233
• NCBI Accession #: NP_000236.2
• NCBI GenBank Nucleotide #: NM_000245.3
• UniProt Accession #: P08581; O60366; Q12875; Q9UDX7; Q9UPL8; A1L467; B5A932; E7EQ94
• Molecular Weight: 85,745 Da
• NCBI Official Full Name: hepatocyte growth factor receptor isoform b preproprotein
• NCBI Official Synonym Full Names: MET proto-oncogene, receptor tyrosine kinase
• NCBI Official Symbol: MET
• NCBI Official Synonym Symbols: HGFR; AUTS9; RCCP2; c-Met; DFNB97
• NCBI Protein Information: hepatocyte growth factor receptor
• UniProt Protein Name: Hepatocyte growth factor receptor
• UniProt Gene Name: MET
• UniProt Synonym Gene Names: HGF receptor; SF receptor

NCBI Description

This gene encodes a member of the receptor tyrosine kinase family of proteins and the product of the proto-oncogene MET. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that are linked via disulfide bonds to form the mature receptor. Further processing of the beta subunit results in the formation of the M10 peptide, which has been shown to reduce lung fibrosis. Binding of its ligand, hepatocyte growth factor, induces dimerization and activation of the receptor, which plays a role in cellular survival, embryogenesis, and cellular migration and invasion. Mutations in this gene are associated with papillary renal cell carcinoma, hepatocellular carcinoma, and various head and neck cancers. Amplification and overexpression of this gene are also associated with multiple human cancers. [provided by RefSeq, May 2016]

Uniprot Description

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling plays a role in gastrulation, development and migration of muscles and neuronal precursors, angiogenesis and kidney formation. In adults, participates in wound healing as well as organ regeneration and tissue remodeling. Promotes also differentiation and proliferation of hematopoietic cells. May regulate cortical bone osteogenesis ().