Goat Octamer Binding Transcription Factor 1 ELISA Kit | OBTF1 elisa kit

Goat Octamer Binding Transcription Factor 1 ELISA Kit

Cat. Num. AAA753276

• Reactivity: Goat
• Synonyms: Octamer Binding Transcription Factor 1; Goat Octamer Binding Transcription Factor 1 ELISA Kit; OBTF1 elisa kit

• Reactivity: Goat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This OBTF1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat OBTF1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for OBTF1 elisa kit

Principle of the assay: OBTF1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-OBTF1 antibody and an OBTF1-HRP conjugate. The assay sample and buffer are incubated together with OBTF1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the OBTF1 concentration since OBTF1 from samples and OBTF1-HRP conjugate compete for the anti-OBTF1 antibody binding site. Since the number of sites is limited, as more sites are occupied by OBTF1 from the sample, fewer sites are left to bind OBTF1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OBTF1 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 418016
• NCBI Official Full Name: octamer binding transcription factor 1

Uniprot Description

POU4F1: Probable transcription factor which may play a role in the regulation of specific gene expression within a subset of neuronal lineages. May play a role in determining or maintaining the identities of a small subset of visual system neurons. Belongs to the POU transcription factor family. Class- 4 subfamily.

Protein type: Transcription factor; Cell development/differentiation; DNA-binding

Chromosomal Location of Human Ortholog: 13q31.1

Cellular Component: neuron projection; nuclear chromatin

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding

Biological Process: transcription from RNA polymerase II promoter; trigeminal nerve development; central nervous system neuron differentiation; positive regulation of apoptosis; negative regulation of transcription from RNA polymerase II promoter; habenula development; cell migration in hindbrain; peripheral nervous system neuron development; proprioception during equilibrioception; regulation of neurogenesis; synaptogenesis; axonogenesis; suckling behavior; neuron fate specification; positive regulation of transcription from RNA polymerase II promoter; mesoderm development; sensory system development; negative regulation of neuron apoptosis