Rabbit MERS-CoV Nucleocapsid Polyclonal Antibody | anti-NP-CoV antibody

MERS-CoV Nucleocapsid Protein Antibody, Rabbit PAb, Antigen Affinity Purified

Cat. Num. AAA8110592

• Reactivity: MERS-CoV
• Applications: Western Blot, ELISA
• Purity: Protein A & Antigen Affinity
• Synonyms: MERS-CoV Nucleocapsid; Polyclonal Antibody; MERS-CoV Nucleocapsid Protein Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-coronavirus NP Antibody; Anti-coronavirus Nucleocapsid Antibody; Anti-coronavirus Nucleoprotein Antibody; Anti-cov np Antibody; Anti-ncov NP Antibody; Anti-novel coronavirus Nucleoprotein Antibody; Anti-NP Antibody; Anti-Nucleocapsid Antibody; Anti-Nucleoprotein Antibody; anti-NP-CoV antibody

• Host: Rabbit
• Reactivity: MERS-CoV
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: The antibody reacts with MERS-CoV (NCoV/Novel coronavirus) Nucleocapsid protein (NP protein).
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-NP-CoV antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:1000-1:5000; ELISA: 1:5000-1:10,000; Antibody's applications haven't been validated with corresponding virus positive samples.
• Immunogen: Recombinant MERS-CoV (NCoV/Novel coronavirus) Nucleoprotein/NP protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant MERS-CoV (NCoV/Novel coronavirus) Nucleoprotein/NP. Nucleoprotein specific IgG was purified by MERS-CoV (NCoV/Novel coronavirus) Nucleoprotein/NP affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-Novel coronavirus (HCoV-EMC/2012) Nucleoprotein / NP rabbit polyclonal antibody at 1:1000 dilutionSample:Novel coronavirus (HCoV-EMC/2012) Nucleoprotein / NPLane A: 10ngLane B: 5ngLane C: 2ngSecondaryGoat anti Rabbit IgG (H+L)/HRP at 0.4ug/ml..Performed under reducing conditions.)

Related Product Information for anti-NP-CoV antibody

Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a tool.

References

1.Van Boheemen S, et al. (2012), MBio. 3(6):e00473-12. Bisht H. et al., 2004, Proc Natl Acad Sci. 101 (17): 6641-6. Li W. et al., 2005, Science. 309 (5742): 1864-8.

NCBI and Uniprot Product Information