Mouse MERS-CoV Nucleocapsid Monoclonal Antibody | anti-NP-CoV antibody

MERS-CoV Nucleocapsid Antibody, Mouse MAb

Cat. Num. AAA8110590

• Reactivity: MERS-CoV
• Applications: Western Blot, ELISA
• Purity: Protein A
• Synonyms: MERS-CoV Nucleocapsid; Monoclonal Antibody; MERS-CoV Nucleocapsid Antibody; Mouse MAb; Anti-coronavirus NP Antibody; Anti-coronavirus Nucleocapsid Antibody; Anti-coronavirus Nucleoprotein Antibody; Anti-cov np Antibody; Anti-ncov NP Antibody; Anti-novel coronavirus Nucleoprotein Antibody; Anti-NP Antibody; Anti-Nucleocapsid Antibody; Anti-Nucleoprotein Antibody; anti-NP-CoV antibody

• Host: Mouse
• Reactivity: MERS-CoV
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: 10
• Specificity: The antibody reacts with MERS-CoV (NCoV/Novel coronavirus) Nucleocapsid protein (NP protein).
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-NP-CoV antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:1000-1:5000; Antibody's applications haven't been validated with corresponding virus positive samples.
• Immunogen: Recombinant MERS-CoV (NCoV/Novel coronavirus) Nucleoprotein/NP protein
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant MERS-CoV (NCoV/Novel coronavirus) Nucleoprotein/NP. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-MERS-CoV (NCoV / Novel coronavirus) Nucleoprotein / NP mouse monoclonal antibody at 1:1000Sample:MERS-CoV (NCoV / Novel coronavirus) Nucleoprotein / NPLane A: 20ngLane B: 5ngLane C: 2ngSecondaryRabbit Anti-Mouse IgGF (ab)2/HRP at 1/10000 dilution.Performed under reducing conditions.)

Related Product Information for anti-NP-CoV antibody

Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a tool.

References

1.Van Boheemen S, et al. (2012), MBio. 3(6):e00473-12. Bisht H. et al., 2004, Proc Natl Acad Sci. 101 (17): 6641-6. Li W. et al., 2005, Science. 309 (5742): 1864-8.

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