Rat Adenylate cyclase type 6 (ADCY6) ELISA Kit | ADCY6 elisa kit

Rat Adenylate cyclase type 6 (ADCY6) ELISA Kit

Cat. Num. AAA7216286

• Gene Names: ADCY6; AC6
• Reactivity: Rat
• Synonyms: Adenylate cyclase type 6 (ADCY6); Rat Adenylate cyclase type 6 (ADCY6) ELISA Kit; ADCY6 elisa kit

• Reactivity: Rat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 5.0-100ng/mL
• Sensitivity: 0.1ng/ml
• Intended Uses: This ADCY6 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat ADCY6. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ADCY6 elisa kit

Principle of the assay: ADCY6 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ADCY6 antibody and an ADCY6-HRP conjugate. The assay sample and buffer are incubated together with ADCY6-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADCY6 concentration since ADCY6 from samples and ADCY6-HRP conjugate compete for the anti-ADCY6 antibody binding site. Since the number of sites is limited, as more sites are occupied by ADCY6 from the sample, fewer sites are left to bind ADCY6-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADCY6 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 10947061
• NCBI GeneID: 112
• NCBI Accession #: NP_066193.1
• NCBI GenBank Nucleotide #: NM_020983.2
• Molecular Weight: 124,865 Da
• NCBI Official Full Name: adenylate cyclase type 6 isoform b
• NCBI Official Synonym Full Names: adenylate cyclase 6
• NCBI Official Symbol: ADCY6
• NCBI Official Synonym Symbols: AC6
• NCBI Protein Information: adenylate cyclase type 6; adenylyl cyclase 6; ATP pyrophosphate-lyase 6; adenylate cyclase type VI; ca(2+)-inhibitable adenylyl cyclase
• UniProt Protein Name: Adenylate cyclase type 6
• Protein Family: Adenylate cyclase
• UniProt Gene Name: ADCY6
• UniProt Synonym Gene Names: KIAA0422
• UniProt Entry Name: ADCY6_HUMAN

NCBI Description

This gene encodes adenylate cyclase 6, which is a membrane-associated enzyme and catalyzes the formation of the secondary messenger cyclic adenosine monophosphate (cAMP). The expression of this gene is found in normal thyroid and brain tissues, as well as some tumors; and its expression is significantly higher in one hyperfunctioning thyroid tumor than in normal thyroid tissue. Alternative splicing generates 2 transcript variants. [provided by RefSeq, Jul 2008]

Uniprot Description

ADCY6: a membrane-associated enzyme that catalyzes the formation of the secondary messenger cyclic adenosine monophosphate (cAMP). Expressed in normal thyroid and brain tissues, as well as some tumors; its expression is significantly higher in one hyperfunctioning thyroid tumor than in normal thyroid tissue. Alternative splicing generates 2 transcript variants.

Protein type: Nucleotide Metabolism - purine; Transporter; Transporter, aquaporin family; Lyase; Adenylyl cyclase; Membrane protein, integral; EC 4.6.1.1; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 12q12-q13

Cellular Component: membrane; integral to membrane; plasma membrane; cilium

Molecular Function: protein binding; metal ion binding; protein kinase binding; ATP binding; adenylate cyclase activity

Biological Process: epidermal growth factor receptor signaling pathway; synaptic transmission; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; phospholipase C activation; water transport; activation of protein kinase A; dopamine receptor signaling pathway; energy reserve metabolic process; innate immune response; renal water homeostasis; cAMP biosynthetic process; G-protein signaling, adenylate cyclase inhibiting pathway; signal transduction; transmembrane transport; G-protein signaling, adenylate cyclase activating pathway

Disease: Lethal Congenital Contracture Syndrome 8