Rabbit anti-Human Meprin beta Polyclonal Antibody | anti-MEP1B antibody

Anti-Meprin beta Antibody, Rabbit Polyclonal

Cat. Num. AAA8102059

• Reactivity: Human
• Applications: Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: Meprin beta; Polyclonal Antibody; Anti-Meprin beta Antibody; Rabbit Polyclonal; MEP1B/Meprin beta Antibody; Rabbit PAb; Antigen Affinity Purified; meprin A; beta; anti-MEP1B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human MEP1B/Meprin beta
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-MEP1B antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Human MEP1B/Meprin beta
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human MEP1B/Meprin beta, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-MEP1B rabbit polyclonal antibody at 1:500 dilutionLane A: A431 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:84 kDaObserved band size:105 kDa)

Related Product Information for anti-MEP1B antibody

MEP1B (Meprin A Subunit Beta) is a Protein Coding gene. This gene encodes the beta subunit. Targeted disruption of this gene in mice affects embryonic viability, renal gene expression profiles, and distribution of the membrane-associated alpha subunit in the kidney and intestine. Meprins are cell membrane, oligomeric metalloendopeptidases composed of two distinct but evolutionarily related subunits, alpha, and beta. MEP1A is mapped to the short arm of chromosome 6 by the use of radiation and somatic cell hybrids. More specifically, it is localized between the centromere and GSTA2 in 6p11-p12. MEP1B mapped to chromosome 18, by the use of somatic cell hybrids, in 18q12.2-q12.3, proximal to the TTR/PALB gene. Diseases associated with MEP1B include Powassan Encephalitis and Deafness, Autosomal Dominant 31.

NCBI and Uniprot Product Information

• NCBI GI #: 815890920
• NCBI GeneID: 4225
• NCBI Accession #: NP_001295100.1
• NCBI GenBank Nucleotide #: NM_001308171.1
• UniProt Accession #: Q16820; Q670J1; B7ZM35; B9EGL6
• Molecular Weight: 79,571 Da
• NCBI Official Full Name: meprin A subunit beta isoform 2
• NCBI Official Synonym Full Names: meprin A subunit beta
• NCBI Official Symbol: MEP1B
• NCBI Protein Information: meprin A subunit beta
• UniProt Protein Name: Meprin A subunit beta
• Protein Family: Meprin
• UniProt Gene Name: MEP1B

NCBI Description

Meprins are multidomain zinc metalloproteases that are highly expressed in mammalian kidney and intestinal brush border membranes, and in leukocytes and certain cancer cells. They are involved in the hydrolysis of a variety of peptide and protein substrates, and have been implicated in cancer and intestinal inflammation. Mature meprins are oligomers of evolutionarily related, but separately encoded alpha and/or beta subunits. Homooligomers of alpha subunit are secreted, whereas, oligomers containing the beta subunit are plasma membrane-bound. This gene encodes the beta subunit. Targeted disruption of this gene in mice affects embryonic viability, renal gene expression profiles, and distribution of the membrane-associated alpha subunit in kidney and intestine. [provided by RefSeq, Oct 2011]

Uniprot Description

Membrane metallopeptidase that sheds many membrane-bound proteins. Exhibits a strong preference for acidic amino acids at the P1' position. Known substrates include: FGF19, VGFA, IL1B, IL18, procollagen I and III, E-cadherin, KLK7, gastrin, ADAM10, tenascin-C. The presence of several pro-inflammatory cytokine among substrates implicate MEP1B in inflammation. It is also involved in tissue remodeling due to its capability to degrade extracellular matrix components.