Sheep anti-Mouse MEPE, ED Polyclonal Antibody | anti-Mepe antibody
MEPE, ED (Matrix Extracellular Phosphoglycoprotein, Osteoblast/Osteocyte Factor 45, OF45)
Gene Names
Mepe; Of45
Reactivity
Mouse
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
MEPE; ED; Polyclonal Antibody; ED (Matrix Extracellular Phosphoglycoprotein; Osteoblast/Osteocyte Factor 45; OF45); Anti -MEPE; anti-Mepe antibody
Host
Sheep
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes the extracellular domain of mouse MEPE.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Concentration
~0.2 mg/ml (after reconstitution) (varies by lot)
Applicable Applications for anti-Mepe antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: Western Blot: 1ug/ml.
ELISA (direct): 0.5-1ug/ml
Dilution: Western Blot: 1ug/ml.
ELISA (direct): 0.5-1ug/ml
Immunogen
Recombinant protein corresponding to aa17-433 at the etracellular domain of mouse MEPE ezpressed in NSO cells (ak70342). Species Sequence Homomlogy: Rat; 77%, human; 57%
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-Mepe antibody
MEPE (matrix extracellular phosphoglycoprotein; also OF45) is a 56-58kD member of the SIBLING protein family. It is expressed by osteocytes, osteoblasts, odontoblasts and chondrocytes. MEPE is found both intra- and extracellularly. Within the cell, it binds to CHK1. This protects CHK1 from degradation, and the cell from DNA damage. Extracellularly, it binds to Phex, which protects it from cathepsin B proteolysis. Undegraded, it promotes bone vascularization and blocks bone resorption. Following cleavage after Arg410 (in mouse), a 2kD ASARM fragment is generated that, when phosphorylated, blocks mineralization. Mature mouse MEPE is 417aa in length. It contains an RGD motif and ASARM domain (aa416-433). In addition to its 57kD form, 70kD, 42-45kD and 30-35kD forms of mouse MEPE have been reported. Whether they are cleavage forms or products of glycosylation is unclear. Over aa147-433, mouse MEPE shares 77% and 57% aa identity with rat and human MEPE, respectively.
Product Categories/Family for anti-Mepe antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
matrix extracellular phosphoglycoprotein with ASARM motif
NCBI Official Synonym Full Names
matrix extracellular phosphoglycoprotein with ASARM motif (bone)
NCBI Official Symbol
Mepe
NCBI Official Synonym Symbols
Of45
NCBI Protein Information
matrix extracellular phosphoglycoprotein with ASARM motif; osteoregulin
Protein Family
Research Articles on Mepe
Similar Products
Product Notes
The Mepe (Catalog #AAA627449) is an Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase. The MEPE, ED (Matrix Extracellular Phosphoglycoprotein, Osteoblast/Osteocyte Factor 45, OF45) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MEPE, ED can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: Western Blot: 1ug/ml. ELISA (direct): 0.5-1ug/ml. Researchers should empirically determine the suitability of the Mepe for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MEPE, ED, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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