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Immunohistochemistry (IHC) (Anti-MEPCE antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Goat MEPCE Polyclonal Antibody | anti-MEPCE antibody

Goat Polyclonal to Human MEPCE

Gene Names
MEPCE; BCDIN3
Reactivity
Bat, Gibbon, Gorilla, Human, Monkey, Rat
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
Immunoaffinity Purified
Synonyms
MEPCE; Polyclonal Antibody; Goat Polyclonal to Human MEPCE; Anti-MEPCE Antibody (Internal) IHC-plus; BCDIN3; Bin3 homolog; Methylphosphate capping enzyme; Human MEPCE; anti-MEPCE antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Bat, Gibbon, Gorilla, Human, Monkey, Rat
Clonality
Polyclonal
Specificity
Human MEPCE.
Purity/Purification
Immunoaffinity Purified
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Concentration
0.5 mg/ml (varies by lot)
Sequence Length
689
Applicable Applications for anti-MEPCE antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes
ELISA (1:2000), IHC-P (5 ug/ml), WB (1:2000, 1 - 3 ug/ml)
Target Species
Human
Immunogen Description
Synthetic peptide KQQRKFQYGNYCK from an internal region of human MEPCE (NP_062552.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Elephant, Bat (100%); Mouse, Rat, Hamster, Panda, Dog, Horse, Pig, Opossum (92%).
Immunogen Type
Synthetic peptide
Immunogen
MEPCE antibody was raised against synthetic peptide KQQRKFQYGNYCK from an internal region of human MEPCE (NP_062552.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Elephant, Bat (100%); Mouse, Rat, Hamster, Panda, Dog, Horse, Pig, Opossum (92%).
Antigen Modification
Internal
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-MEPCE antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC) (Anti-MEPCE antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Antibody (1 ug/ml) staining of Rat Testis lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB) (Antibody (1 ug/ml) staining of Rat Testis lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
24,950 Da
NCBI Official Full Name
7SK snRNA methylphosphate capping enzyme isoform A
NCBI Official Synonym Full Names
methylphosphate capping enzyme
NCBI Official Symbol
MEPCE
NCBI Official Synonym Symbols
BCDIN3
NCBI Protein Information
7SK snRNA methylphosphate capping enzyme; bin3 homolog; bin3, bicoid-interacting 3, homolog; bicoid-interacting protein 3 homolog
UniProt Protein Name
7SK snRNA methylphosphate capping enzyme
UniProt Gene Name
MEPCE
UniProt Synonym Gene Names
BCDIN3; MePCE; Bin3 homolog
UniProt Entry Name
MEPCE_HUMAN

Uniprot Description

BCDIN3: a CDK9-associated enzyme that forms the distinctive gamma-methylphosphate cap structure of 7SK, a noncoding RNA that regulates CDK9 activity. An adenosyl-L-methionine-dependent methyltransferase that adds a methylphosphate cap at the 5'-end of 7SK snRNA, leading to its stabilization. Component of the 7SK snRNP complex that contains P-TEFb (composed of CDK9 and CCNT1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Expressed in chronic myeloid leukemia cells, adrenal gland, brain, cerebellum, kidney, lung, mammary gland and testis. Weakly or not expressed in other tissues. Contains a Bin3 domain found in a number of protein methyltransferases that modify RNA-binding proteins.

Protein type: RNA processing; Transcription regulation; EC 2.1.1.-; EC 2.1.1.; Methyltransferase

Chromosomal Location of Human Ortholog: 7q22.1

Molecular Function: S-adenosylmethionine-dependent methyltransferase activity; RNA methyltransferase activity

Biological Process: snRNA metabolic process; snRNA modification; RNA methylation; negative regulation of transcription from RNA polymerase II promoter

Research Articles on MEPCE

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Product Notes

The MEPCE mepce (Catalog #AAA248883) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat Polyclonal to Human MEPCE reacts with Bat, Gibbon, Gorilla, Human, Monkey, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MEPCE can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA). ELISA (1:2000), IHC-P (5 ug/ml), WB (1:2000, 1 - 3 ug/ml). Researchers should empirically determine the suitability of the MEPCE mepce for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MEPCE, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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