Sheep anti-Human Membrane AssociatedGuanalyate Kinase GKWW domain (MAGI) Polyclonal Antibody | anti-MAGI antibody

Membrane AssociatedGuanalyate Kinase GKWW domain (MAGI)

Cat. Num. AAA395584

• Gene Names: MAGI3; MAGI-3; dJ730K3.2; RP4-730K3.1
• Reactivity: Human
• Applications: Western Blot
• Purity: Ammonium Sulfate Precipitation
• Synonyms: Membrane AssociatedGuanalyate Kinase GKWW domain (MAGI); Polyclonal Antibody; Sheep anti-Membrane-associated guanylate cyclase of inverted orientation-3; Guanylate Kinase-WW domains; anti-MAGI antibody

• Host: Sheep
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Ammonium Sulfate Precipitation
• Form/Format: Provided as solution in phosphate buffered saline with 0.08% sodium azide
• Sequence Length: 1481

• Applicable Applications for anti-MAGI antibody: Western Blot (WB)
• Immunogen: Recombinant protein corresponding to the guanylate kinase-WW domain of MAGI-3.
• Preparation and Storage: Product should be stored at -20 degree C. Aliquot to avoid freeze/thaw cycles

Western Blot/Blotting

(Western blot analysis)

Related Product Information for anti-MAGI antibody

Membrane-associated guanylate kinase with inverted orientation (MAGI3) is a novel, 130 kDa guanylate kinase which is part of a family of multi-PDZ domain containing guanylate kinases. These guanylate kinases are localized to epithelial cell tight junctions. MAGI3 and PTEN/MMAC cooperate to modulate the kinase activity of AKT/protein kinase B (PKB). MAGI3 positions the PTEN/MMAC phosphatase to specific subcellular locations that are involved with the regulation of cell proliferation and survival. MAGI3, as well as other proteins in this family, are made up of an N-terminal guanylate kinase domain, followed by a WW domain, named for two conserved tryptophan residues and first identified as two repeats in mouse YAP65, which is then followed by 6 PDZ domains.

Product Categories/Family for anti-MAGI antibody

Polyclonal Antibody; Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 218505835
• NCBI GeneID: 260425
• NCBI Accession #: NP_001136254.1
• NCBI GenBank Nucleotide #: NM_001142782.1
• UniProt Accession #: Q9H2V6; Q5TCQ8; Q5TCR0; Q9H5Y8; Q9HBC4; Q9HCD8; Q5TCQ9
• Molecular Weight: 130
• NCBI Official Full Name: membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3 isoform 1
• NCBI Official Synonym Full Names: membrane associated guanylate kinase, WW and PDZ domain containing 3
• NCBI Official Symbol: MAGI3
• NCBI Official Synonym Symbols: MAGI-3; dJ730K3.2; RP4-730K3.1
• NCBI Protein Information: membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3; membrane-associated guanylate kinase-related 3; membrane-associated guanylate kinase inverted 3
• UniProt Protein Name: Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3
• UniProt Gene Name: MAGI3
• UniProt Synonym Gene Names: KIAA1634; MAGI-3
• UniProt Entry Name: MAGI3_HUMAN

Uniprot Description

Function: Acts as a scaffolding protein at cell-cell junctions, thereby regulating various cellular and signaling processes. Cooperates with PTEN to modulate the kinase activity of AKT1. Its interaction with PTPRB and tyrosine phosphorylated proteins suggests that it may link receptor tyrosine phosphatase with its substrates at the plasma membrane. In polarized epithelial cells, involved in efficient trafficking of TGFA to the cell surface. Regulates the ability of LPAR2 to activate ERK and RhoA pathways. Regulates the JNK signaling cascade via its interaction with FZD4 and VANGL2. Ref.1

Subunit structure: Interacts with ADRB1, FZD4, FZD7, PTPRB, TGFA and VANGL2. Interacts with unidentified tyrosine phosphorylated proteins

By similarity. Interacts with BAI1, LPAR2/EDG4, GRIN2B and PTEN. In case of infection, interacts with HTLV TAX1 protein, possibly affecting the transformation ability of TAX1. Does not interact with HTLV TAX2 or TAX3 proteins. In case of infection, interacts with HPV type 16 and HPV type 18 E6 protein. Ref.1 Ref.9 Ref.10 Ref.11 Ref.12 Ref.14 Ref.15

Subcellular location: Cell membrane; Peripheral membrane protein. Cell junction › tight junction. Nucleus

By similarity. Note: Concentrates in specific sites at the plasma membrane and in the nucleus. In epithelial cells, it localizes at tight junctions

By similarity. Ref.1 Ref.8

Tissue specificity: Widely expressed. Ref.1

Post-translational modification: Ubiquitinated following interaction with HPV E6 protein, leading to its degradation by the proteasome. Degradation is independent of E6AP ubiquitin ligase complex. Ref.9 Ref.15

Miscellaneous: MAGI3 PDZ domains are used to design peptide ligands that bind and inhibit PDZ domains.

Sequence similarities: Belongs to the MAGUK family.Contains 1 guanylate kinase-like domain.Contains 6 PDZ (DHR) domains.Contains 2 WW domains.

Sequence caution: The sequence BAB15479.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence BAB15479.1 differs from that shown. Reason: Frameshift at position 1373.