Rabbit anti-Human MAGI3 Polyclonal Antibody | anti-MAGI3 antibody

MAGI3 Antibody (C-Term)

Cat. Num. AAA9215751

• Gene Names: MAGI3; MAGI-3; dJ730K3.2
• Reactivity: Human
• Applications: Western Blot
• Synonyms: MAGI3; Polyclonal Antibody; MAGI3 Antibody (C-Term); Purified Rabbit Polyclonal Antibody (Pab); Membrane-associated guanylate kinase; WW and PDZ domain-containing protein 3; Membrane-associated guanylate kinase inverted 3; MAGI-3; KIAA1634; anti-MAGI3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This MAGI3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1330-1360 amino acids from human MAGI3.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 1481

• Applicable Applications for anti-MAGI3 antibody: Western Blot (WB)
• Application Notes: WB ~~ 1:2000
• Antigen Source: Human
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Anti-MAGI3 Antibody (C-Term) at 1:2000 dilution + human brain lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 166 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-MAGI3 antibody

Acts as a scaffolding protein at cell-cell junctions, thereby regulating various cellular and signaling processes. Cooperates with PTEN to modulate the kinase activity of AKT1. Its interaction with PTPRB and tyrosine phosphorylated proteins suggests that it may link receptor tyrosine phosphatase with its substrates at the plasma membrane. In polarized epithelial cells, involved in efficient trafficking of TGFA to the cell surface. Regulates the ability of LPAR2 to activate ERK and RhoA pathways. Regulates the JNK signaling cascade via its interaction with FZD4 and VANGL2.

Product Categories/Family for anti-MAGI3 antibody

Signal Transduction

References

Wu Y.,et al.J. Biol. Chem. 275:21477-21485(2000). Franklin J.L.,et al.Exp. Cell Res. 303:457-470(2005). Gregory S.G.,et al.Nature 441:315-321(2006). Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases. Nagase T.,et al.DNA Res. 7:273-281(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 218505835
• NCBI GeneID: 260425
• NCBI Accession #: NP_001136254.1
• NCBI GenBank Nucleotide #: NM_001142782.1
• UniProt Accession #: Q5TCQ9; Q5TCQ8; Q5TCR0; Q9H2V6; Q9H5Y8; Q9HBC4; Q9HCD8
• Molecular Weight: 165608
• NCBI Official Full Name: membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3 isoform 1
• NCBI Official Synonym Full Names: membrane associated guanylate kinase, WW and PDZ domain containing 3
• NCBI Official Symbol: MAGI3
• NCBI Official Synonym Symbols: MAGI-3; dJ730K3.2
• NCBI Protein Information: membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3
• UniProt Protein Name: Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3
• Protein Family: Membrane-associated guanylate kinase, WW and PDZ domain-containing protein
• UniProt Gene Name: MAGI3
• UniProt Synonym Gene Names: KIAA1634; MAGI-3
• UniProt Entry Name: MAGI3_HUMAN

NCBI Description

MAGI3: a protein of the MAGUK family of proteins. A peripheral membrane protein found at cell-cell junctions. Contains one guanylate kinase, two WW, and four PDZ domains. Cooperates with PTEN to modulate the kinase activity of AKT1. Its interaction with PTPRB and tyrosine phosphorylated proteins suggests that it may link receptor tyrosine phosphatase with its substrates at the plasma membrane. In polarized epithelial cells, involved in efficient trafficking of TGFA to the cell surface. Regulates the ability of LPAR2 to activate ERK and RhoA pathways. Regulates the JNK signaling cascade via its interaction with FZD4 and VANGL2. Interacts with ADRB1, FZD4, FZD7, PTPRB, TGFA and VANGL2. Interacts with BAI1, LPAR2/EDG4, GRIN2B and PTEN. In case of infection, interacts with HTLV TAX1 protein, possibly affecting the transformation ability of TAX1. Does not interact with HTLV TAX2 or TAX3 proteins. Interacts with HPV type 16 and HPV type 18 E6 protein. Ubiquitinated following interaction with HPV E6 protein, leading to its degradation by the proteasome. Degradation is independent of E6AP ubiquitin ligase complex. Four alternatively spliced human isoforms have been described.

Protein type: Membrane protein, peripheral; Kinase, other

Chromosomal Location of Human Ortholog: 1p13.2

Cellular Component: tight junction; membrane; plasma membrane; nucleus

Molecular Function: guanylate kinase activity; protein binding; frizzled binding; protein complex scaffold; ATP binding

Biological Process: positive regulation of JNK activity; viral reproduction; apoptosis; GDP metabolic process; GMP metabolic process

Uniprot Description

MAGI3: a protein of the MAGUK family of proteins. A peripheral membrane protein found at cell-cell junctions. Contains one guanylate kinase, two WW, and four PDZ domains. Cooperates with PTEN to modulate the kinase activity of AKT1. Its interaction with PTPRB and tyrosine phosphorylated proteins suggests that it may link receptor tyrosine phosphatase with its substrates at the plasma membrane. In polarized epithelial cells, involved in efficient trafficking of TGFA to the cell surface. Regulates the ability of LPAR2 to activate ERK and RhoA pathways. Regulates the JNK signaling cascade via its interaction with FZD4 and VANGL2. Interacts with ADRB1, FZD4, FZD7, PTPRB, TGFA and VANGL2. Interacts with BAI1, LPAR2/EDG4, GRIN2B and PTEN. In case of infection, interacts with HTLV TAX1 protein, possibly affecting the transformation ability of TAX1. Does not interact with HTLV TAX2 or TAX3 proteins. Interacts with HPV type 16 and HPV type 18 E6 protein. Ubiquitinated following interaction with HPV E6 protein, leading to its degradation by the proteasome. Degradation is independent of E6AP ubiquitin ligase complex. Four alternatively spliced human isoforms have been described.

Protein type: Kinase, other; Membrane protein, peripheral

Chromosomal Location of Human Ortholog: 1p13.2

Cellular Component: membrane; nucleus; plasma membrane; tight junction

Molecular Function: ATP binding; frizzled binding; guanylate kinase activity; protein binding; protein complex scaffold

Biological Process: apoptosis; GDP metabolic process; GMP metabolic process; positive regulation of JNK activity; viral reproduction